甲巯咪唑联合地黄总苷对毒性弥漫性甲状腺肿模型小鼠甲状腺激素水平和脱碘酶的影响  

Effect of Methimazole Combined with Rehmannia Glutinosaon on Thyroid Hormone and Deiodinases in Mouse Model of Graves' Disease

作　　者：李彬[1] 黄琳[1] 赵建国[1] 阮剑[1] LI Bin;HUANG Lin;ZHAO Jianguo;RUAN Jian(Department of Breast and Thyroid Surgery,the First Hospital of Wuhan City,Wuhan 430022,China)

机构地区：[1]武汉市第一医院甲乳外科,武汉430022

出　　处：《医药导报》2018年第10期1185-1188,共4页Herald of Medicine

摘　　要：目的探索地黄总苷和甲巯咪唑对毒性弥漫性甲状腺肿(GD)小鼠甲状腺激素水平和脱碘酶的影响。方法采用注射左旋甲状腺素钠的方法制备GD小鼠模型。造模成功后,将小鼠分成模型对照组、甲巯咪唑组、甲巯咪唑+地黄总苷组,每组各12只。另设空白对照组12只。甲巯咪唑组灌胃给予甲巯咪唑2 mg·kg^(-1),甲巯咪唑+地黄总苷组灌胃给予甲巯咪唑2 mg·kg^(-1)+地黄总苷120 mg·kg^(-1),空白对照组和模型对照组灌胃给予等量0.9%氯化钠溶液,连续21 d。测定小鼠体质量和摄食量;治疗结束后,测量小鼠心率,检测三碘甲腺原氨酸(T_3)、游离三碘甲腺原氨酸(FT_3)、甲状腺素(T_4)、游离甲状腺素(FT_4)等4种甲状腺激素水平和Ⅰ型脱碘酶(ID1)、Ⅱ型脱碘酶(ID2)、Ⅲ型脱碘酶(ID3)的活性。结果与空白对照组比较,模型对照组小鼠体质量和ID3活性显著降低(P<0.05),T_3、T_4、FT_3、FT_4水平、ID1活性、摄食量和心率均显著升高(P<0.05);与甲巯咪唑组比较,甲巯咪唑+地黄总苷组T_3、T_4、FT_3、FT_4水平、ID1活性均显著降低,ID3活性显著升高(P<0.05),ID2活性差异无统计学意义(P>0.05)。与模型对照组比较,两组小鼠ID2活性均显著降低(P<0.05)。结论甲巯咪唑联合地黄总苷能更加有效地改变脱碘酶的活性,下调甲状腺激素,达到较好治疗效果。Objective To explore the effect of methimazole combined with rehmannia glutinosaon thyroid hormone levels iodothyronine deiodinases in mice with Graves'diseases(GD).Methods Injection with sinistral thyroxine sodium to establish GD mouse model.After the model established,the mice were divided into model control group,methimazole group and methimazole combined with rehmannia glutinosaon group,12 mice in blank control group.The methimazole group and themethimazole combined with rehmannia glutinosaon group were respectively treated with methimazole and themethimazole combined with rehmannia glutinosaon,and then the body weight and food intake were measure.After treatment,the heart rate,and the levels of total triiodothyronine(T 3),free triiodothyronine(FT 3),thyroxine(T 4),free thyroxine(FT 4),and the activity of typeⅠdeiodinases(ID1),typeⅡdeiodinases(ID2),and typeⅢdeiodinases(ID3)in mice were detected.Results Compared with the blank control group,the weight and the activity of ID3 in the model control group were significantly reduced(P<0.05),and the levels of T 3,T 4,FT 3,FT 4,and the activity of ID1,food intake,and heart rate were significantly increased(P<0.05).Compared with the methimazole group,the levels of T 3,T 4,FT 3,FT 4,the activity of ID1 were significantly decreased in the methimazole combined with rehmannia glutinosaon group,the activity of ID3 was significantly increased(P<0.05).There was no significant difference in the activity of ID2 between the two treatment groups(P>0.05).But compared with model control group,the activity of ID2 in two treated group were significantly decreased(P<0.05).Conclusion The methimazole combined with rehmannia glutinosaon effectively changes the activity of iodine enzyme,and decreases the level of thyroid hormone.The therapeutic effect of methimazole combined with rehmannia glutinosaon in GD is significantly better than that of the methimazole.

关 键 词：甲巯咪唑 地黄总苷 毒性弥漫性甲状腺肿 甲状腺激素 脱碘酶 

分 类 号：R977.14[医药卫生—药品] R965[医药卫生—药学]