高效液相色谱法同时测定银蒲解毒片中7种主要成分的含量  

Simultaneous Determination of Seven Component Contents of Yinpujiedu Tablets by HPLC

作　　者：俞学炜[1] 丁荣敏[1] 吴芝园[1] YU Xuewei;DING Rongmin;WU Zhiyuan(School of Pharmacy,Suzhou Vocational Health College,Suzhou 215009,China)

机构地区：[1]苏州卫生职业技术学院药学院,苏州215009

出　　处：《医药导报》2018年第10期1247-1250,共4页Herald of Medicine

基　　金：苏州卫生职业技术学院2015年院级科研立项课题(SZWZY201501)

摘　　要：目的建立高效液相色谱(HPLC)法同时测定银蒲解毒片中新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C和迷迭香酸的含量。方法采用ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.8%磷酸溶液(B)为流动相,梯度洗脱(0~10 min,5%A→10%A;>10~20 min,10%A;>20~30 min,10%A→20%A;>30~45 min,20%A→30%A),流速1.0 m L·min^(-1),柱温35℃,波长327 nm。结果新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C和迷迭香酸进样量分别在0.203~4.06,0.328~6.56,0.318~6.36,0.219~4.38,0.122~2.44,0.239~4.78,0.179~3.58μg范围内与峰面积呈良好线性响应;平均回收率分别为99.04%,100.10%,101.99%,101.65%,99.82%,100.13%,100.25%,RSD分别为1.07%,1.24%,0.60%,0.97%,0.89%,1.67%,0.69%(n=6)。结论该方法对7种成分进行同步测定,准确可靠,重复性好,可用于银蒲解毒片的质量控制。Objective To establish a content determination method of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,and rosmarinic acid in Yinpujiedu tablets by HPLC.Methods A ZORBAX SB-C 18 chromatographic column(250 mm×4.6 mm,5μm)was used;mobile phase was acetonitrile(A)-0.8%phosphoric acid solution(B),the eluting gradient was as follows:0-10 min,5%A→10%A;>10-20 min,10%A;>20-30 min,10%A→20%A;>30-45 min,20%A→30%A;flow rate was 1.0 mL·min-1;column temperature was 35℃,and wavelength was 327 nm.Results Neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,and rosmarinic acid samples were in the ranges of 0.203-4.06,0.328-6.56,0.318-6.36,0.219-4.38,0.122-2.44,0.239-4.78,0.179-3.58μg,respectively,and linearly corresponds well with the AUC of peaks;The average recovery rates(n=6)were 99.04%,100.10%,101.99%,101.65%,99.82%,100.13%and 100.25%,respectively,with RSD were 1.07%,1.24%,0.60%,0.97%,0.89%,1.67%and 0.69%,respectively.Conclusion The method determines seven components simultaneously with high accuracy and reproducibility,and can be used for quality control of Yinpujiedu tablets.

关 键 词：银蒲解毒片 新绿原酸 绿原酸 隐绿原酸 异绿原酸 迷迭香酸 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]