SeMet/CS纳米复合微球的制备、缓释性能及抑制肿瘤活性  

Preparationand sustained-release property of SeMet/CS microsphere and its inhibitory effects on cancer cells

作　　者：冉青 商龙臣 吴少尉 李光大[3] 刘信平 RAN Qing;SHANG Long-Chen;WU Shao-Wei;LI Guang-Da;LIU Xin-Ping(College of Chemistry and Environment Engineering,Hubei Minzu University,Enshi 445000,China;College of Biological Science and Technology,Hubei Minzu University,Enshi 445000,China;College of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]湖北民族学院化学与环境工程学院,恩施445000 [2]湖北民族学院生物科学与技术学院,恩施445000 [3]河南科技大学医学技术与工程学院,洛阳471003

出　　处：《四川大学学报（自然科学版）》2018年第5期1057-1066,共10页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(21461009;81402225;21565013)

摘　　要：研究SeMet硒化物的可控缓释系统.以CS为壁材、SeMet为药物模型,采用乳化交联法制备SeMet/CS纳米复合微球,以单因素实验和正交试验优化制备工艺,结合原子荧光光谱、IR、热重分析及SEM对产物的形貌、结构及性能进行评价和表征,并对其体外缓释和抑制人乳腺癌细胞MCF-7细胞生长等性能进行研究.结果表明:最优工艺条件(0.1%的投药量、2%的交联剂用量、50℃交联10min)制备的SeMet/CS微球,形貌优良,包封率和载药量分别为31.94%和0.54%,体外缓释硒的性能良好,对MCF-7细胞的生长抑制率随微球缓释时间增加而增加.SeMet/CS缓释硒复合微球有效避免了硒的突释效应,实现了硒剂量的把控,可作为硒化药物或硒补充剂应用于医疗、食品和精细化工等相关行业.SeMet/CS Nanocomposite microspheres were prepared by using emulsion crosslinking with SeMet as a core material and chitosan(CS)as a wall material.The work in this paper focused on the indicators of microspheric form,drug-loading rate,entrapment efficiency and slow-release property using the single-factor and orthogonal test to optimize the preparation technology.During the processes,a scanning electron microscope,a Fourier infrared spectrometer,a thermal analyzer and double-channel atomic fluorescence were applied for detection,analysis and characterization.Furthermore,in vitro tests,such as for the selenium-releasing property and anticancer activity,were studied for the prepared selenium microsphere.The data indicated that the SeMet/CS microsphere prepared under the optimum conditions of 0.1%dosage and 2%dosage of crosslinking agent were crosslinked at 50℃for 10 min.The obtained SeMet/CS microspheres exhibited superior features,with an excellent morphology and an embedding ratio and drug loading of 31.94%and 0.59%,respectively.They displayed excellent sustained-release capability in an SBF model.Regarding anticancer activity,they showed a significant inhibitory effect on MCF-7 human breast cancer cells,and there was a positively correlated between the inhibition rate and the content of selenium in the sustained-release liquid.The SeMet/CS microsphere can effectively avoid a burst effect for selenium,allowing a controlled selenium dose.Thus,it can be used in selenide or selenium supplements for further application in medical,food and fine chemical industries.

关 键 词：SeMet/CS微球 制备表征 性能 

分 类 号：O627.6[理学—有机化学]