神经干细胞提取及培养方法的体会  

Experience in the extraction and culture of neural stem cells

作　　者：吴增[1] 董贤慧 李媛[1] 靳晓飞 周晓红[2] 高维娟[2] WU Zeng;DONG Xian-hui;LI Yuan;JIN Xiao-fei;ZHOU Xiao-hong;GAO Wei-juan(Department of Pathophysiology,Chengde Medical College,Chengde 067000,China;Hebei University of Chinese Medicine,Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease)

机构地区：[1]承德医学院病理生理学教研室,067000 [2]河北中医学院,河北省心脑血管病中医药防治重点实验室

出　　处：《天津医药》2018年第8期881-885,共5页Tianjin Medical Journal

基　　金：河北省政府资助临床医学优秀人才培养和基础课题研究项目([2017]46号-2);河北省中医药管理局科研计划项目(2015007);河北省高等学校科学技术研究项目(ZD2016101);河北省科技基础条件建设项目(179677114D);河北省硕士研究生创新资助项目(CXZZSS2017136)

摘　　要：神经干细胞提取及体外培养的方法有多种,但采用不同方法所得到的神经干细胞的活力与稳定性均不同。通过对提取和体外培养SD胎鼠大脑皮质神经干细胞的过程进行思考,总结出神经干细胞的提取步骤、提取要点、培养基的选择依据、接种密度的选择、培养方法、换液方法、传代时机、传代方法,最终获得大量活力和稳定性均较高的神经干细胞,并将其应用于相关方面的基础研究。There are many ways to extract and culture neural stem cells in vitro,but the viability and stability of neural stem cells obtained by different methods are different.By thinking about the process of extracting and culturing neural stem cells in vitro from the cerebral cortex of SD fetal rats,we summarized extraction steps,the main points of extraction,the selection basis of culture medium,selection of inoculation density,cultural method,methods of solution changing,passage time and passage methods.At last a large number of neural stem cells with high vitality and stability have been obtained and applied to the basic research of neural stem cells.

关 键 词：干细胞 神经元 培养基 连续传代 细胞提取 悬浮培养法 

分 类 号：R331[医药卫生—人体生理学]