荧光探针在细胞成像领域的研究进展  

Research progress of fluorescent probes in cell imaging

作　　者：杨媛[1] 马拉毛草 马恒昌[1] YANG Yuan;MA Lamaocao;MA Hengchang(Key Laboratory of Macromolecule Materials,Ministry of Education, College of Chemistry and Chemical Engineering,Northwest Normal University, Key Laboratory of Polymer Materials of Gansu Province,Lanzhou 730070,China;Shaw Library,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学化学与化工学院,生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,兰州730070 [2]西北师范大学逸夫图书馆,兰州730070

出　　处：《功能材料》2018年第9期9031-9037,共7页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(21764012)。

摘　　要：荧光成像技术是生物医学领域的重要研究手段,可对目标分子进行原位实时的监测,且这种方法具有无损伤、高特异性和高灵敏度,以及能在细胞水平获得更高的分辨率等优势。近年来,荧光材料在离子分子识别、医学诊断、生物分子检测以及生物成像等领域显示出了重要的应用价值,因此受到越来越多的化学和材料工作者的重视。综述了碳纳米材料、半导体量子点、稀土金属、有机荧光小分子、聚合物荧光纳米颗粒几种常见不同类型的荧光探针材料在细胞成像领域的应用,介绍了其发射波长、荧光量子产率、生物相容性、光稳定性、细胞毒性以及遗传毒性等特性。设计并合成发射波长较长、Stokes位移大、生物相容性好、光稳定性好、廉价的荧光探针将是荧光成像技术的主要研究方向。Fluorescence imaging has emerged as an important research tool in the field of biomedicine because it not only has the advantages of high specificity and sensitivity,but also can monitor the amount,localization,and movement of biomolecules at the cellular level without damage.In recent years,fluorescent materials have found widespread applications in diverse fields,including molecular ion recognition,medical diagnosis,biomolecule detection and biological imaging.Therefore,fluorescent materials have been paid more and more attention by chemical and material workers.In this review,the applications in the field of bioimaging of several different types of fluorescent probe materials,including carbon nanomaterials,semiconductor quantum dots,rare earth metals,organic fluorescent molecules,polymer fluorescent nanoparticles and fluorescent proteins,are summarized as well as their wavelength,fluorescence quantum yield,biocompatibility,photostability,cytotoxicity and genotoxicity.The future of the field will mainly depend on designing and synthesizing bio-probe with long emission wavelength,large Stokes Shift,good biocompatibility,excellent light stability and low cost.

关 键 词：荧光材料 荧光探针 细胞成像 

分 类 号：O63[理学—高分子化学]