铁酸锌纳米颗粒对序批式反应器性能及微生物群落的影响  

Effects of ZnFe_2O_4 Nanoparticles on the Performance and Microbial Community of Sequencing Batch Reactor

作　　者：董俊伟 马丙瑞 李珊珊 万一平 于娜玲 赵长坤 李志伟[1,2] 高孟春[1,2] DONG Jun-Wei;MA Bing-Rui;LI Shan-Shan;WAN Yi-Ping;YU Na-Ling;ZHAO Chang-Kun;LI Zhi-Wei;GAO Meng-Chun(The Key Laboratory of Marine Environmental Science and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100 China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2018年第A01期153-161,共9页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(51178437)资助~~

摘　　要：本文研究了铁酸锌纳米颗粒(ZnFe_2O_4 NPs)浓度变化对序批式反应器(SBR)性能、微生物酶活性和微生物群落的影响。结果表明:0~50mg/L的ZnFe_2O_4 NPs对COD和NH_4^+-N的去除未产生影响,出水NO_2^--N浓度接近于0mg/L,而出水NO_3^--N浓度在3.89~5.63mg/L之间变化。随着进水,ZnFe_2O_4 NPs浓度从0mg/L增加到50mg/L,活性污泥的比耗氧速率(SOUR)、比氨氧化速率(SAOR)、比亚硝酸盐还原速率(SNIRR)、脱氢酶(DHA)、氨单加氧酶(AMO)和亚硝酸盐还原酶(NIR)的活性逐渐降低。与进水ZnFe_2O_4 NPs浓度为0mg/L时相比,活性氧(ROS)产生量和乳酸脱氢酶(LDH)释放量的变化表明,ZnFe_2O_4 NPs对活性污泥中微生物有一定毒性效应。ZnFe_2O_4 NPs的浓度变化导致微生物群落种类和相对丰度在门和属水平上发生变化。该研究结果为评价ZnFe_2O_4 NPs对污水生物处理系统的潜在影响提供可靠的理论基础和技术依据。The performance,microbial enzymatic activity and microbial community of a sequencing batch reactor(SBR)were investigated at different Zinc ferrite Nanoparticles(ZnFe 2O 4 NPs)concentrations.The results indicated that the ZnFe 2O 4 NPs concentration at 0~50 mg/L had no adverse effects on the COD and NH+4-N removal.The effluent NO-2-N concentration is close to zero,while the effluent NO-3-N concentration ranged from 3.89 to 5.63 mg/L.The specific oxygen utilization rate(SOUR),specific ammonia oxidation rate(SAOR),specific nitrite reduction rate(SNIRR),dehydrogenase activity(DHA),ammonia monooxygenase(AMO)and nitrite reductase(NIR)gradually reduced with an increase in ZnFe 2O 4 NPs concentration from 0 to 50 mg/L.Compared to 0 mg/L ZnFe 2O 4 NPs,The reactive oxygen species(ROS)production and lactate dehydrogenase(LDH)release demonstrated that ZnFe 2O 4 NPs NPs led to the toxicity to activated sludge.High throughput sequencing indicated that ZnFe 2O 4 NPs could obviously affect the microbial composition and relative abundance of activated sludge at phyla and genus level.The results provide a reliable theoretical basis and technical basis for evaluating the potential impact of ZnFe 2O 4 NPs on biological wastewater treatment systems.

关 键 词：ZnFe2O4NPs 序批式反应器(SBR) 活性污泥 微生物酶活性 高通量测序 

分 类 号：X78[环境科学与工程—环境工程]