覆土层益生菌恶臭假单胞菌TK3对双孢蘑菇的促生作用  

Addition of Pseudomonas putida TK3 into the casingsoil topromotegrowth of Agaricus bisporus

作　　者：王琳[1] 魏启舜 周影[1] 赵荷娟 陈悦[2] 李辉信[2] 郭成宝[1] WANG Lin;WEI Qishun;ZHOU Ying;ZHAO Hejuan;CHEN Yue;LI Huixin;GUO Chengbao(Nanjing Institute of Agricultural Science in Jiangsu Hilly Area,Nanjing,Jiangsu 210046,China;College of resources and environmental sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

机构地区：[1]江苏丘陵地区南京农业科学研究所,江苏南京210046 [2]南京农业大学资源与环境科学学院,江苏南京210095

出　　处：《食用菌学报》2018年第3期23-29,107,共8页Acta Edulis Fungi

基　　金：江苏省农业科技自主创新资金[CX(17)3017]

摘　　要：为研究恶臭假单胞菌(Pseudomonas putida)TK3对双孢蘑菇(Agaricus bisporus)的促生作用,用红色荧光蛋白rfp基因标记菌株TK3,获得的转化子TK3(rfp)在紫外光照射下发出红色荧光,且转入的rfp基因未对TK3细胞产生毒性。将TK3(rfp)与双孢蘑菇菌丝进行共培养,发现TK3(rfp)可促进双孢蘑菇菌丝的生长并促进菌丝扭结成团。在覆土层中喷施TK3(rfp)菌液,20d后覆土层中细菌总数和TK3(rfp)数量随着TK3(rfp)喷施量的增加而增加,说明TK3(rfp)能在覆土层中定殖,当喷施浓度为每克覆土107cfu时,与对照相比,双孢蘑菇子实体产量增加24.9%。In order to study the effect of Pseudomonas putida TK3 on A.bisporus,a red fluorescence protein gene rfp was transformed into TK3 to generate TK3(rfp).TK3(rfp)can emit red fluorescence under UV light and the heterologous protein was non-toxic to the host.When TK3(rfp)was co-cultured with A.bisporus on culture medium,it stimulated mycelial growth as well as twisting of mycelia.Then TK3(rfp)suspension was sprayed into the casing soil at different inocula.The results showed that 20 days after spray inoculation,total number of bacteria and TK3(rfp)in the casing soil increased as spray inoculum increased,suggesting that TK3(rfp)colonized in the casing soil.Compared with the blank control,fruiting body yield under 10 7 cfu TK3(rfp)per gram of casing soil inoculum increased by 24.9%.

关 键 词：恶臭假单胞菌 红色荧光蛋白 双孢蘑菇 覆土 促生 

分 类 号：S646[农业科学—蔬菜学]