弱磁场对樟芝液态发酵菌丝体细胞膜流动性的影响  

Effect of Low-intensityMagnetic Field on Cell Membrane Fluidityof Taiwanofungus camphoratus Mycelia in Liquid Fermentation

作　　者：郭丹钊[1] 赵杨 葛璐 马海乐[1,2] 李振江[3] GUO Danzhao;ZHAO Yang;GE Lu;MA Haile;LI Zhenjiang(School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China;Instistute of Agro-production processing Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013,China;State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing,Jiangsu 210009,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学农产品加工工程研究院,江苏镇江212013 [3]南京工业大学材料化学工程国家重点实验室,江苏南京210009

出　　处：《食用菌学报》2018年第3期42-48,共7页Acta Edulis Fungi

基　　金：国家自然科学基金项目(31301544);南京工业大学材料化学工程国家重点实验室开放课题基金资助项目(KL15-12);江苏高校优势学科建设工程资助项目

摘　　要：采用荧光偏振法测定弱磁场对樟芝(Taiwanofungus camphoratus)ATCC 200183菌丝体细胞膜流动性的影响。以1,6-二苯基-1,3,5-己三烯(1,6-dipheny-1,3,5-hexatriene,DPH)作为荧光探针标记樟芝菌丝体细胞膜,优化DPH荧光标记条件,测定不同弱磁场强度(30、60、90、120mT和180mT)下菌丝体细胞膜荧光各向异性及荧光偏振度的变化。结果表明,DPH标记细胞膜激发波长和发射波长分别324nm和453nm,DPH最佳标记温度和时间分别为30℃和45min;磁场强度为30mT时,菌丝体细胞膜荧光偏振度、荧光各向异性与对照组(未粘贴铷铁硼磁片)相比差异不显著(P>0.05);磁场强度为60、90、120mT和180mT时,菌丝体细胞膜荧光偏振度、荧光各向异性显著低于对照组(P<0.05);90、120、180mT处理组间差异不显著(P>0.05)。在供试弱磁场强度下,菌丝体生物量的变化趋势与细胞膜流动性变化趋势相似,在磁场强度为90mT时菌丝体生物量达到最大值(4.230g/L),显著高于不加磁场的对照处理(3.119g/L)。The effect of low intensity magnetic field on the cell membrane fluidity of Taiwainofungus camphoratus was studied using the fluorescence polarization method.Using 1,6-dipheny-1,3,5-hexatriene(DPH)as the fluorescence probe to label mycelial cell membrane of T.camphoratus,the labeling condition was first optimized and then degree of fluorescence polarization and fluorescence anisotropy of mycelia cell membrane were measured under different intensities of magnetic fields(30,60,90,120,and 180 mT).The results showed that the excitation and the emission wavelength of DPH labeled cell membrane were 324 nm and 453 nm,respectively.The optimal DPH labeling temperature and time were 30℃and 45 min.As the strength of the magnetic field increased,degree of fluorescence polarization and fluorescence anisotropy decreased.Compared with the control group,the degree of polarization and the fluorescence anisotropy under 30 mT magnetic field were not significantly different.On the other hand,60,90,120 and 180 mT magnetic fields resulted in a significantly difference in degree of fluorescence polarization and fluorescence anisotropy compared to the control.There was no difference in degree of fluorescence polarization and fluorescence anisotropy among the three magnetic fields(90,120 and 180 mT).When treated under low intensity magnetic fields,mycelial biomass showed a consistent kinetics with that of the cell membrane fluidity,and peaked under 90 mT with a biomass yield of 4.23 g/L,which was significantly greater than the control without any magnetic field treatment.

关 键 词：标记温度 标记时间 菌丝体生物量 荧光偏振度 荧光各向异性 

分 类 号：S567.39[农业科学—中草药栽培]