超高效液相色谱-串联质谱法检测鸡肉中6种大环内酯类药物残留量  

Determination of Six Macrolides Residues in Chicken by Ultra-high Serformance Liquid Chromatography-tandem Mass Spectrometry

作　　者：罗燕 LUO Yan(Shenzhen Agricultural Products Quality and Safety Inspection and Testing Center,Shenzhen 518005,Guangzhou China)

机构地区：[1]深圳市农产品质量安全检验检测中心,广东深圳518005

出　　处：《饲料博览》2018年第9期52-56,共5页Feed Review

摘　　要：建立鸡肉中6种大环内酯类药物的高效液相色谱-串联四级杆质谱(UPLC-MS/MS)残留分析方法。样本在乙腈水溶液提取下经PRiME HLB固相萃取柱净化,氮吹浓缩后定容过膜上机检测。在1.00～50.0μg·L^(-1)线性范围内,红霉素、替米考星、泰乐菌素、林可霉素、吉他霉素和螺旋霉素的标准曲线相关系数(r)>0.999,方法检出限<0.95μg·kg^(-1),空白样品加标回收率范围为75.8%～110.7%,相对标准偏差<11.50%,适用于可食动物肌肉红霉素、替米考星、泰乐菌素、林可霉素、吉他霉素和螺旋霉素残留的检测。The method for the residue analysis of six macrolides in chicken was established by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were purified by PRIME HLB solid phase extraction column under the extraction of acetonitrile water.After drying and concentrating,the samples were reused and tested over the membrane.In the linear range of 1.00 microgram per liter,and the detection limit of the method was less than 0.95 microgram per liter,the correlation coefficient of the standard curve of erythromycin,tilmicosin,tylosin,lincomycin,kitasamycin and spiramycin was higher than 0.999,the detection limit were less than 0.95 microgram per liter.The recovery of standard addition range of the blank sample was 75.8%-10.7%,and the relative standard deviation was less than 11.50%.This method was suitable for the detection of edible animal muscle oleandomycin,clindamycin,josamycin residues.

关 键 词：红霉素 替米考星 泰乐菌素 林可霉素 吉他霉素 螺旋霉素 高效液相色谱-串联质谱 

分 类 号：S831[农业科学—畜牧学] TS207.5[农业科学—畜牧兽医]