基于完全酸水解组成糖特征的几种法定基源麻黄多糖鉴别研究  

Identification of Polysaccharides from Several Kinds of Legal Base Source of Ephedra Based on the Chacteristics of Compositional Saccharides

作　　者：梁军[1] 孙黎明[1] 宋艳 王天龙 夏永刚[1] 匡海学[1] LIANG Jun;SUN Li-ming;SONG Yan;WANG Tian-long;XIA Yong-gang;KUANG Hai-xue(Key Laboratory of Chinese Materia Medica,Ministry of Education,Heilongjang Key Laboratory of TCM and Natural Medicine Pharmacodynamic Material Bases,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040

出　　处：《天然产物研究与开发》2018年第9期1477-1483,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81803809;81303215);全国优秀博士学位论文作者专项基金(201367);中国博士后第61批科学基金面上项目(2017M611409)

摘　　要：草麻黄多糖具有重要的免疫抑制作用,因此对几种法定基源麻黄属多糖进行鉴别是一项重要的工作。本研究采用PMP柱前衍生超高效液相色谱-串联质谱(UPLC-MS/MS)技术分析草麻黄、中麻黄、木贼麻黄和麻黄根多糖的完全酸水解单糖组成,考察其组成糖种类和含量变化,运用主成分分析多元统计分析方法,对源自草麻黄、中麻黄、木贼麻黄和麻黄根的二十批几种法定基源麻黄多糖实现了区分和辨别。甘露糖(1)、氨基葡萄糖(2)、半乳糖醛酸(8)和葡萄糖(9)在区分草麻黄多糖与麻黄根、中麻黄和木贼麻黄等三种麻黄多糖中起到了重要作用,半乳糖(10)在区分木贼麻黄多糖与草麻黄、中麻黄和麻黄根等三种麻黄多糖中起到了重要作用。PMP柱前衍生UPLC-MS/MS技术结合多元统计学分析方法,通过完全酸水解组成糖种类和含量的变化规律进行草麻黄、中麻黄、木贼麻黄和麻黄根多糖鉴别研究,该方法稳定可行,且重现性好,可为麻黄属多糖的质量评价提供有力参考。Because polysaccharides from Ephedra sinica stem have reported to possess important immunosuppressive activity,it is significant to identify and discriminate polysaccharides from several kinds of legal base source of Genus Ephedra.This study realized the discrimination of polysaccharides from Ephedra sinica Stapf,E.intermdia Schrenk et C.A.Mey.,E.equisetina Bge and Badix Ephedra employing UPLC-MS/MS techniques based on changeable rules of compositional monosaccharides by full acid hydrolysis.The results showed 20 batches of Ephedra polysaccharides was successfully discriminated using UPLC-MS/MS coupled with multivariate statistics method,such as principal component analysis(PCA).These Ephedra polysaccharides were attributed to different species and plant parts.Man(1),GlcN(2),GalUA(8)and Glc(9)played an important role in classifying between E.sinica polysaccharides and polysaccharides from E.intermdia、E.equisetina and Badix Ephedra,while Gal(10)played an important role in classifying between E.equisetina polysaccharides and polysaccharides from E.intermdia,E.sinica and Badix Ephedra.This method with the advantages of high stability and repeatability,could achieve a quality evaluation of plant polysaccharides from three kinds of legal base source of Genus Ephedra.

关 键 词：麻黄多糖 完全酸水解 组成糖 超高效液相色谱-串联质谱 主成分分析 

分 类 号：R284.1[医药卫生—中药学]