一株平沙绿僵菌的鉴定及生防应用潜力评价  

Identification of Metarhizium pingshaense and assessing potentiality of bio-control application

作　　者：杨帆[1] 刘春来[1] 王爽[1] 刘亮 李新民[1] YANG Fan;LIU Chunlai;WANG Shuang;LIU Liang;LI Xinmin(Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences,Scientific Observing and Experimental Station of Crop Pest in Harbin,Ministry of Agriculture,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院植物保护研究所,农业部哈尔滨作物有害生物科学观测实验站,哈尔滨150086

出　　处：《植物保护》2018年第5期199-205,共7页Plant Protection

基　　金：黑龙江省农业科学院农业科技创新工程(2014QN009);国家重点研发计划(2017YFD0201108)

摘　　要：利用大蜡螟诱集法,从黑龙江地区不同类型土样中筛选分离到一株虫生真菌FZZ菌株,经形态学及18SrDNA ITS序列分析鉴定为平沙绿僵菌Metarhizium pingshaense(KY419576)。通过对平沙绿僵菌FZZ菌株的生物学特性、其对小菜蛾Plutella xylostella 2龄幼虫的致病性及对7种植物病原菌的抑菌活性的研究结果表明,FZZ菌株菌丝生长的最适温度为25℃,产孢的最适温度范围为20~25℃,30℃孢子萌发率最高,但48h也仅为48%。浓度为1.0×109个/mL的孢子悬浮液处理小菜蛾2龄幼虫,5d后校正累计侵染率为78.33%。FZZ菌株对7株植物病原菌菌丝生长均有不同程度的抑制作用,对水稻稻瘟病菌Magnaporthe oryzae菌丝抑制率最高,达45.45%,对大豆根腐病菌Fusarium oxysporum的抑制率最低,为36.31%。FZZ菌株发酵滤液对番茄灰霉病菌Botrytis cinerea有较强抑菌活性,抑菌带宽直径达2.93cm,对其余6种病原菌未表现出抑制效果。此结果对筛选高效平沙绿僵菌及扩大其生防范围具有重要意义。An entomopathogenic fungal strain FZZ isolated from infected Galleria mellonella larvae was identified as Metarhizium pingshaense by morphological and molecular methods.Study on biological characteristics of the isolate showed that the optimum temperature for mycelium growth was 25℃.The range of optimum temperature for conidia production was from 20℃to 25℃.The most suitable temperature for conidia germination was 30℃with the germination rate of 48%at 48 h.The virulence of M.pingshaense strain FZZ against the second instar larvae of Plutella xylostella was investigated in the laboratory.The corrected accumulative infection rate reached 78.33%after 5 days treatment at a concentration of 1.0×10 9 conidia/mL suspension.The inhibition activity of strain FZZ against 7 species of plant pathogenic fungi showed that the highest inhibition rate on mycelium growth of Magnaporthe oryzae was 45.45%and the lowest inhibition rate on mycelium growth of Fusarium oxysporum was 36.31%.Only the fermented filtrated of strain FZZ had inhibition against mycelium growth of Botrytis cinerea,with the inhibition zone diameter of 2.93 cm.No inhibition effects were found in FZZ on other six pathogens.The results showed the great importance for screening M.pingshaense with high antagonistic activity and enlarging its biological control targets.

关 键 词：平沙绿僵菌 生物学特性 致病力 抑菌作用 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]