人体肠道菌群对人参皂苷Rg_3、白头翁皂苷D代谢转化的影响  

Effects of human intestinal flora on the metabolic transformations of ginsenoside Rg_3 and pulsatilla saponin D

作　　者：严桐[1] 易婷[1] 郑婷婷[1] YAN Tong;YI Ting;ZHENG Ting-ting(The Third People’s Hospital of Changzhou,Changzhou 213001,China)

机构地区：[1]常州市第三人民医院,江苏常州213001

出　　处：《中成药》2018年第9期1902-1909,共8页Chinese Traditional Patent Medicine

摘　　要：目的考察人体肠道菌群对人参皂苷Rg_3、白头翁皂苷D代谢转化的影响。方法分别采集肝癌患者与健康人体的新鲜粪便,经处理后分别与人参皂苷Rg_3、白头翁皂苷D进行厌氧共孵育,UPLC-Q-TOF/MS法鉴定其肠菌孵育后的代谢产物差异,UPLC-QQQ-MS法检测原型及其代谢产物含有量随孵育时间的变化。结果肝癌患者与健康人体肠道菌群的人参皂苷Rg_3孵育液中均鉴定出了人参皂苷Rh2(脱糖基产物)、原人参二醇;肝癌患者肠道菌群的白头翁皂苷D孵育液中鉴定出了白头翁皂苷D脱鼠李糖产物、白头翁皂苷D脱葡萄糖产物、白头翁皂苷D羟基化产物、常春藤皂苷元,但健康人体肠道菌群的白头翁皂苷D孵育液中鉴定出了白头翁皂苷D脱鼠李糖产物、白头翁皂苷D脱葡萄糖产物、白头翁皂苷D脱鼠李糖和葡萄糖产物、白头翁皂苷D甲基化产物、常春藤皂苷元。人参皂苷Rg_3、白头翁皂苷D在肝癌患者肠道菌群中的代谢速率显著低于健康人体,人参皂苷Rh2和常春藤皂苷元在肝癌患者肠道菌群中的生成速率显著低于健康人体。结论在肝癌状态下,人体肠道菌群变化对人参皂苷Rg_3、白头翁皂苷D代谢转化有显著影响。AIM To investigate the effects of human intestinal flora on the metabolic transformations of ginsenoside Rg3 and pulsatilla saponin D.METHODS The fresh feces collected from liver cancer patients and healthy human were incubated with intestinal bacteria and ginsenoside Rg3 and pulsatilla saponin D,respectively,under anaerobic condition.The metabolites differences were identified by UPLC-Q-TOF-MS,and the quantitative changes of ginsenoside Rg3,pulsatilla saponin D and their metabolites with incubation time were detected by UPLC-QQQ-MS.RESULTS Ginsenoside Rh2(de-glycosylated product)and protopanaxadiol were observable in ginsenoside Rg3 intestinal bacteria incubation solution of the liver cancer patients and healthy human.De-rhamnose product,deglucose product and hydroxylated product of pulsatilla saponin D,and hederagenine were identified in the pulsatilla saponin D intestinal bacteria incubation solution of the liver cancer patients.But De-rhamnose product,de-glucose product,de-rhamnose and glucose product and methylation product of pulsatilla saponin D,and hederagenine were found in the pulsatilla saponin D intestinal bacteria incubation solution of the healthy human.However,the metabolic rate of ginsenoside Rg3 and pulsatilla saponin D in the intestinal bacteria of the liver cancer patients were significantly lower than that of the healthy human.Meanwhile,the generation rate of ginsenoside Rh2 and hederagenine in the intestinal bacteria of the liver cancer patients was significantly lower than that of the healthy human.CONCLUSION The abnormal human intestinal flora due to liver cancer obviously affects the metabolic transformations of ginsenoside Rg3 and pulsatilla saponin D.

关 键 词：人体肠道菌群 人参皂苷RG3 白头翁皂苷D 肝癌 UPLC-Q-TOF/MS UPLC-QQQ-MS 

分 类 号：R966[医药卫生—药理学]