肝清宁合剂对四氯化碳致肝硬化大鼠的影响  

Effects of Ganqingning Mixture on rats with carbon tetrachloride-induced liver cirrhosis

作　　者：赵凯丽 许立拔[1] 邓佳欣 梁飞 黄增琼[1] 曾凡艳[2] ZHAO Kai-li;XU Li-ba;DENG Jia-xin;LIANG Fei;HUANG Zeng-qiong;ZENG Fan-yan(College of Pharmacy,Guangxi Medical University,Nanning 530021,China;Tumor Hospital Affiliated to Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西医科大学附属肿瘤医院,广西南宁530021

出　　处：《中成药》2018年第9期1915-1919,共5页Chinese Traditional Patent Medicine

基　　金：广西医科大学青年科学基金项目(GXMUYSF201626);广西医科大学大学生创新创业训练计划项目(201610598028)

摘　　要：目的研究肝清宁合剂对四氯化碳致大鼠肝硬化的影响。方法造模大鼠灌胃给予肝清宁合剂(5.4、10.8、21.5 g/kg)或复方鳖甲软肝片(0.8 g/kg)连续4周后,HE、Masson染色检查肝组织病理变化,全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、总蛋白(TP)、总胆红素(TBIL)水平,ELISA法测定血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原肽(PⅢNP)、Ⅳ型胶原(CⅣ)水平,酶标法和紫外分光光度法测定肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(HYP)水平。结果与模型组比较,肝清宁合剂组ALT、AST、TBIL、HA、LN、PⅢNP、CⅣ水平显著降低(P<0.05,P<0.01),ALB、TP水平显著升高(P<0.05,P<0.01),HYP、MDA水平显著降低(P<0.01),SOD水平显著升高(P<0.05)。同时,肝组织病理损伤减轻,肝纤维化间隔变薄或减少。结论肝清宁合剂对四氯化碳致肝硬化大鼠具有良好的治疗作用,可能与改善肝脏功能和纤维化指标、促进肝组织自愈、增强机体抗氧化能力有关。AIM To study the effects of Ganqingning Mixture on rats with carbon tetrachloride-induced liver cirrhosis.METHODS Rat models intragastrically administered with four-week Ganqingning Mixture(5.4,10.8,21.5 g/kg)or four-week Compound Biejia Ruangan Tablets(0.8 g/kg)had their pathological changes of liver tissue checked by HE staining and Masson staining,their serum levels of alanine transaminase(ALT),aspartate transaminase(AST),albumin(ALB),total protein(TP)and total bilirubin(TBIL)determined by automatic biochemistry analyzer,their serum levels of hyaluronic acid(HA),laminin(LN),typeⅢprocollagen(PⅢNP)and typeⅣcollagen(CⅣ)detected by ELISA,their hepatic levels of superoxide dismutase(SOD),malondialdehyde(MDA)and hydroxyproline(HYP)measured by enzyme labeling method and UV spectrophotometry.RESULTS Compared with the model group,Ganqingning Mixture groups were identified to be with markedly decreased levels of ALT,AST,TBIL,HA,LN,PⅢNP and CⅣ(P<0.05,P<0.01),notably increased levels of ALB and TP(P<0.05,P<0.01),obviously decreased levels of HYP and MDA(P<0.01),significantly increased level of SOD(P<0.05).Meanwhile,improvement in pathological injury of liver tissue,and thinner or less liver fibrotic septa were observed in rats intervened by Ganqingning Mixture.CONCLUSION The therapeutic effects of Ganqingning Mixture on rats with carbon tetrachloride-induced liver cirrhosis suggest its power in improving liver function,liver tissue self-healing and fibrosis management,and enhancing body antioxidant ability.

关 键 词：肝清宁合剂 肝硬化 四氯化碳 

分 类 号：R285.5[医药卫生—中药学]