蒲葵子总黄酮提取工艺的优化及其体外抗肿瘤活性  

Extraction optimization for total flavonoids from Livistona chinensis and the in vitro anti-tumor activity

作　　者：朱丽 黄松[1,2,3] 林吉 赖小平[1,2,3] 张桂芳 贺莲[4] ZHU Li;HUANG Song;LIN Ji;LAI Xiao-ping;ZHANG Gui-fang;HE Lian(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China;Dongguan Municipal Institution for Mathematics and Theoretics Engineering Research for Traditional Chinese Medicine,Guangzhou University of Traditional Chinese Medicine,Dongguan 523808,China;Guangdong Provincial Institution for Mathematics and Theoretics Engineering Research for Traditional Chinese Medicine,Guangzhou 510006,China;Guangdong Food and Drug Vocational College,Guangzhou 510006,China)

机构地区：[1]广州中医药大学,广东广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808 [3]广东省中医药数理工程研究院,广东广州510006 [4]广东食品药品职业学院,广东广州510006

出　　处：《中成药》2018年第9期1959-1964,共6页Chinese Traditional Patent Medicine

基　　金：广东省省级科技计划项目(2013A022100002)

摘　　要：目的优化蒲葵子总黄酮提取工艺,并评价其体外抗肿瘤活性。方法以提取时间、超声功率、乙醇体积分数、液料比为影响因素,总黄酮提取率为评价指标,星点设计-效应面法优化提取工艺。然后,MTT法检测总黄酮对SGC7901、Hep G2、A549细胞的抑制作用。结果最佳条件为提取时间30 min,超声功率80 Hz,乙醇体积分数65%,液料比30∶1,总黄酮提取率11.59%。与阴性对照比较,总黄酮对3种肿瘤细胞均具有显著抑制作用(P<0.05,P<0.01),并呈量效关系,48 h内最大抑制率分别为83.77%、93.33%、74.39%。结论该方法稳定可靠,可用于提取具有较强体外抗肿瘤活性的蒲葵子总黄酮。AIM To optimize the extraction for total flavonoids from Livistona chinensis(Jacq.)R.Br.and to evaluate the in vitro anti-tumor activity.METHODS With extraction time,ultrasound power,ethanol concentration and liquid-solid ratio as influencing factors,total flavonoids extraction rate as an evaluation index,central composite design-response surface method was applied to optimizing the extraction.Afterwards,the inhibition effects of total flavonoids on SGC7901,HepG2,A549 cells were detected by MTT assay.RESULTS The optimal conditions were determined to be 30 min for extraction time,80 Hz for ultrasound power,65%for ethanol concentration,and 30∶1 for liquid-solid ratio,the total flavonoids extraction rate was 11.59%.Compared with negative control,total flavonoids exhibited significant inhibition effects on three tumor cells in a dose-effect manner(P<0.05,P<0.01),with the maximal inhibition rates of 83.77%,93.33%,74.39%within 48 h,respectively.CONCLUSION This stable and reliable method can be used for the extraction for total flavonoids from L.chinensis with strong in vitro anti-tumor activity.

关 键 词：蒲葵子 总黄酮 提取 体外抗肿瘤活性 星点设计-效应面法 MTT法 

分 类 号：R284.2[医药卫生—中药学]