HPLC法同时测定五仁润肠丸中10种成分  

Simultaneous determination of ten constituents in Wuren Runchang Pills by HPLC

作　　者：李智利[1] LI Zhi-li(Institute of Pharmaceutical and Environmental Engineering,Changzhou Vocational Institute of Engineering,Changzhou 213000,China)

机构地区：[1]常州工程职业技术学院制药与环境工程学院,江苏常州213000

出　　处：《中成药》2018年第9期1978-1981,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定五仁润肠丸(桃仁、地黄、陈皮等)中苦杏仁苷、梓醇、麦角甾苷、吉奥诺苷B1、马替诺皂苷、芸香柚皮苷、橙皮苷、川陈皮素、3,5,6,7,8,3',4'-七甲氧基黄酮、橘红素的含有量。方法该药物50%甲醇提取液的分析采用Hydrosphere C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%磷酸,梯度洗脱;体积流量0.9 m L/min;检测波长210、300、330 nm;柱温30℃。结果 10种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.93%~100.17%,RSD 0.77%~1.47%。结论该方法简便准确,重复性好,可用于五仁润肠丸的质量控制。AIM To establish an HPLC method for the simultaneous content determination of amygdalin,catalpol,acteoside,jionoside B1,martinoside,narirutin,hesperidin,nobiletin,3,5,6,7,8,3',4'-heptemthoxyflavone and tangeratin in Wuren Runchang Pills(Persicae Semen,Rehmanniae Radix,Citri reticulatae Pericarpium,etc.).METHODS The analysis of 50%methanol extract of this drug was performed on a 30℃thermostatic Hydrosphere C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,300,330 nm.RESULTS Ten constituents showed good linear relationships within their own ranges(r>0.999 0),whose average recoveries were 96.93%-100.17%with the RSDs of 0.77%-1.47%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Wuren Runchang Pills.

关 键 词：五仁润肠丸 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]