普通小麦–大赖草易位系T5AS-7LrL·7LrS分子细胞遗传学鉴定  

Molecular and Cytogenetic Identification of Triticum aestivum–Leymus racemosus Translocation Line T5AS-7LrL·7LrS

作　　者：王林生 张雅莉 南广慧 WANG Lin-Sheng;ZHANG Ya-Li;NAN Guang-Hui(Key Laboratory of Crop Genetic Improvement and Germplasm Innovation/College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China)

机构地区：[1]河南科技大学农学院/洛阳市作物遗传改良与种质创新重点实验室,河南洛阳471023

出　　处：《作物学报》2018年第10期1442-1447,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31501301);河南省国际合作项目(172102410052);河南省教育厅自然科学研究项目(2011A180011)资助~~

摘　　要：大赖草对赤霉病具有较好的抗性,将大赖草赤霉病抗性基因转入普通小麦,对拓宽小麦赤霉病抗性基础有重要意义。本研究在获得抗赤霉病普通小麦–大赖草异附加系基础上,采用^(60)Co-γ射线(1200Rad,剂量率100Rad min^(-1))处理小麦–大赖草二体附加系DA7Lr,并用处理后的花粉授给去雄的普通小麦中国春,对其M_1代种子根尖细胞有丝分裂中期染色体进行GISH分析,获得1株具有一条普通小麦–大赖草易位染色体的植株,对其自交后代中具有2条易位染色体植株的花粉母细胞减数分裂中期I观察发现,2条易位染色体形成了稳定的环状二价体,表明该植株为纯合体。利用顺序GISH–双色FISH分析,结合C-分带、小麦D组专化探针Oligo-pAs1-2和B组专化探针Oligo-pSc119.2-2,进一步鉴定出该易位系为T5AS-7LrL·7LrS,同时筛选出可追踪该易位系的3个EST-STS分子标记,即BE591127、BQ168298和BE591737。该易位系的育成也为小麦赤霉病遗改良提供了新种质。Leymus racemosus is highly resistant to wheat scab.The transfer of resistance genes from L.racemosus to common wheat(Triticum aestivum)is important for broadening the resistant sources against scab in common wheat.In this study,the pollen of DA7Lr,a T.aestivum–L.racemosus disomic addition line with scab resistance,was irradiated with 60Co-γray at 1200 Rad(100 Rad min?1)before pollinating to emasculated T.aestivum cv.Chinese Spring.One plant with one translocation chromosome was detected in the M1 generation by GISH.This plant was then self-pollinated and the pollen mother cells(PMCs)of the offspring plants with two translocation chromosomes were cytologically observed,and one ring bivalent was found at meiotic metaphase I,indicating that the plant with two translocation chromosomes was one translocation homozygote.The translocation line was proved to be T5AS-7LrL·7LrS by C-banding,and sequential GISH–FISH using Oligo-pAs1-2 and Oligo-pSc119.2-2 as probes.Three EST-STS markers(BE591127,BQ168298,and BE591737)were identified to be able to track the T5AS-7LrL·7LrS line.The translocation line also serves as an resistant source against wheat scab in wheat breeding programs.

关 键 词：普通小麦–大赖草易位系 分子细胞遗传学 赤霉病抗性 60Co-γ射线 

分 类 号：S512.1[农业科学—作物学]