免疫抑制BALB/c小鼠系统性白假丝酵母菌感染生物指标模型的建立  

Establishment of animal model of systemic Candida albicans infection in immunosuppressive BALB/c mice

作　　者：李继红[1] 邓洁华[1] 赵宜乐 王刚生[1] LI Ji-hong;DENG Jie-hua;ZHAO Yi-le;WANG Gang-sheng(The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院,河北石家庄050000

出　　处：《中国感染控制杂志》2018年第10期878-883,共6页Chinese Journal of Infection Control

基　　金：河北省自然科学基金(H2013206316)

摘　　要：目的探讨经腹腔注射白假丝酵母菌建立免疫抑制BALB/c小鼠系统性感染的生物指标模型,为研究白假丝酵母菌感染的致病机制和抗真菌药物的药效学提供相关动物模型。方法对免疫抑制组BALB/c小鼠(腹腔注射环磷酰胺200 mg/kg·d,连续2 d)经腹腔注射白假丝酵母菌增强毒力株0.25 mL(浓度为1×10^(7 )CFU/mL)建立系统性白假丝酵母菌感染模型;取小鼠尾静脉血进行白细胞和中性粒细胞计数,取小鼠组织进行真菌镜检、培养、病理检查以及(1,3)-β-D-葡聚糖检测。结果免疫抑制组与对照组小鼠用药后第4天白细胞计数、中性粒细胞计数、平均体重比较,差异均具有统计学意义(均P<0.05)。白假丝酵母菌感染组生存率为30.00%,对照组生存率为100.00%,两组生存率比较,差异有统计学意义(P<0.05)。对注射真菌后第2~14天死亡小鼠以及第14天存活小鼠进行解剖,发现肺、肝、肾组织出现多处脓肿,以肾组织感染最为显著;小鼠组织真菌直接镜检可见大量菌丝体,组织培养均为白假丝酵母菌,组织病理可见大量菌丝体、炎细胞及组织坏死。白假丝酵母菌感染组肺、肾组织(1,3)-β-D-葡聚糖均增高,与对照组比较,差异均有统计学意义(均P<0.05)。结论本实验方法可以成功建立免疫抑制BALB/c小鼠系统性白假丝酵母菌感染的动物模型。Objective To establish a biological model of systemic infection in immunosuppressed BALB/c mice by intraperitoneal injection of Candida albicans(C.albicans),and provide animal model for studying the pathogenesis of C.albicans infection and pharmacodynamics of antifungal agents.Methods C.albicans infection model of immunosuppressed BALB/c mice(intraperitoneally injected with cyclophosphamide 200 mg/kg·d for consecutive 2 days)was established through intraperitoneal injection of 0.25 mL virulence-enhanced strain of C.albicans(concentration:1×10 7 CFU/mL).Vein blood of mice tail was taken for detecting white blood cell count and neutrophil count,mice tissue were collected for microscopic fungal examination,culture,pathological examination,and(1,3)-β-D-glucan detection.Results There were significant differences in white blood cell count,neutrophil count,and ave-rage body weight between immunosuppressive group and control group on the 4th day after treatment(all P<0.05).The survival rate of C.albicans infection group and control group were 30.00%and 100.00%respectively,difference between two groups was statistically significant(P<0.05).Dissection of mice which died on day 2-14 and survival mice on day 14 after injection of C.albicans found that there were multiple abscess in lung,liver,and kidney tissue,especially kidney infection;a large number of fungal mycelia could be seen by direct microscopic examination of mice tissue,C.albicans was found through tissue culture,histopathology examination showed a large number of mycelia,inflammatory cells and tissue necrosis.The levels of(1,3)-β-D-glucan in lung and kidney tissue of C.albicans infection group were both significantly higher than those of control group(both P<0.05).Conclusion Animal model of systemic C.albicans infection in immunosuppressed BALB/c mice can be successfully established by this method.

关 键 词：白假丝酵母菌 组织真菌学 组织病理学 (1 3)-β-D-葡聚糖 感染 

分 类 号：R379.4[医药卫生—病原生物学]