提高哺乳动物克隆效率的研究进展  

Research Progress on Methods to Improve Mammalian Cloning Efficiency

作　　者：许卫华[1,2] 吴珍芳 石俊松 XU Weihua;WU Zhenfang;SHI Junsong(College of Life Science,Longyan University,Longyan 364012,China;Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan 364012,China;National Engineering Research Center for Breeding Swine Industry,Guangzhou 510642,China;Institute of Wen’s Group,Xinxing 527400,China)

机构地区：[1]龙岩学院生命科学学院,龙岩364012 [2]福建省家畜传染病防治与生物技术重点实验室,龙岩364012 [3]国家生猪种业工程技术研究中心,广州510642 [4]广东省温氏集团研究院,新兴527400

出　　处：《中国畜牧兽医》2018年第9期2516-2523,共8页China Animal Husbandry & Veterinary Medicine

基　　金：福建省自然科学基金资助项目(2015J01620);福建省中青年教师教育科研项目(JA14306);龙岩学院引进人才科研启动经费(LB2014025)

摘　　要：体细胞核移植技术(somatic cell nuclear transfer,SCNT)又称为体细胞克隆技术,在生物医学、遗传修饰动物研究及大家畜的育种和良种扩繁等领域具有重要的理论意义和现实价值,但目前该项技术依然存在如克隆效率低下、克隆动物表型异常等问题。近年来,研究人员通过在培养基中添加小分子药物、选择优势供体细胞系(包括iPS细胞)、优化传统核移植参数、对发育关键基因(如XIST及H3K9me3去甲基化酶等)的靶向遗传调控等途径,探索提高克隆效率的新思路、新工艺和新方法,作者着重对上述研究进展进行简要综述。Somatic cell nuclear transfer(SCNT),also named as somatic cell cloning technology,has vast importance and implications for biomedicine,genetically modified animals research and livestock breeding or expansion of excellent individuals.Currently,mammalian cloning technology remains inefficient and cloned animals usually accompanies various phenotypic defects.In recent years,researchers have been exploring many ideas,techniques and methods to improve cloning efficiency,which include addition of small molecules in culture media,seeking for superior donor cells like iPS cells,optimization of conditioning parameters for nuclear transfer manipulations and genetic regulation of developmentally important genes such as XIST and H3K9me 3 demethylase genes.This paper was aimed to summary above-mentioned progress on cloning efficiency promotion.

关 键 词：哺乳动物 体细胞核移植技术(SCNT) 克隆效率 

分 类 号：S814.8[农业科学—畜牧学]