核酸等温扩增技术在水产病原体快速检测中的应用  

Application of isothermal nucleic acid amplification technologies in rapid detection of aquatic pathogens

作　　者：庞建虎 乔龙亮 朱鹏[1,2] 高威芳 章礼萍 PANG Jianhu;QIAO Longliang;ZHU Peng;GAO Weifang;ZHANG Liping(School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China;Ningbo Institute of Oceanography,Ningbo,Zhejiang 315832,China)

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]宁波海洋研究院,浙江宁波315832

出　　处：《福建农林大学学报（自然科学版）》2018年第5期530-540,共11页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家现代农业产业技术体系建设专项(CARS-47);浙江省公益技术应用研究项目(2017C33133);宁波市科技攻关项目(2017C110003)

摘　　要：有别于费力和费时的传统病原体检测方法,基于靶基因的分子生物学检测方法以其快速和灵敏的特点逐渐成为现代水产病原体检测的首选.在众多分子生物学检测方法中,尤以核酸等温扩增技术备受关注.本文对各种核酸等温扩增技术在水产病原体检测中的应用进行了综述,阐述了各种核酸等温扩增技术的原理,对比分析了各种核酸等温扩增技术的优劣,介绍了该技术在水产病原体检测中的应用现状,展望了该技术的发展趋势,旨在促进我国水产病原体现场快速检测技术的发展.Different from traditional pathogen detection method,molecular biology detection is based on target gene.It becomes the first choice for modern aquatic pathogen detection for its rapid and sensitive characteristics.Among numerous methods,nucleic acid isother^nal amplification technology attracts much attention.In this paper,the application of various isother^nal nucleic acid amplification techniques in the detection of aquatic pathogenic microorganisms was systematically reviewed,together with comparison and analysis on their advantages and disadvantages.This paper is contributed to the establishment of a more complete reference for point-of-care testing for aquatic pathogens in China in the future.

关 键 词：核酸等温扩增 水产病原体 快速检测 

分 类 号：Q789[生物学—分子生物学]