畸型精子症患者精子DNA完整性与年龄及常规精液参数的关系  

Correlation of Sperm DNA Integrity with Age and Routine Semen Parameters in Teratozoospermic Men

作　　者：郭丽媛[1] 周华[1] 王晓蔓[1] 刘敏[1] GUO Li-yuan;ZHOU Hua;WANG Xiao-man;LIU Min(Institute of Obstetrics and Gynecology//Key Lab of Reproduction and Genetics of Guangdong Higher Education Institute,The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China)

机构地区：[1]广州医科大学附属第三医院妇产科研究所//广东省普通高校生殖与遗传重点实验室,广东广州510150

出　　处：《中山大学学报（医学版）》2018年第5期731-735,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省医学科研基金(B2018227)

摘　　要：【目的】探讨畸形精子症患者的精子DNA完整性与年龄及常规精液参数的相关关系。【方法】收集2013年1月至2016年12月在我院生殖中心就诊的255例畸型精子症患者的精液标本,将患者按年龄分为≤30岁、30~35岁和≥35岁3组,按WHO第5版手册进行精液常规分析,采用精子染色质扩散法(SCD)检测精子DNA完整性。【结果】畸形精子症患者精子DNA碎片指数(DFI)与前向运动精子百分率(PR%)、精子总活力呈负相关(P <0.001;P <0.001),与年龄、正常形态精子百分率(NF%)可能存在弱相关关系(P=0.003;P=0.027)。精子浓度、总精子数与精子DFI之间的相关关系无统计学意义(P=0.260;P=0.541)。不同年龄组患者的精子DFI水平差异具有统计学意义(P=0.021);年龄≥35岁组的精子DFI水平显著高于≤30岁组和30~35岁组(P=0.030,P=0.035);而≤30岁组和30~35岁组间的精子DFI水平差异无统计学意义(P=1.000)。年龄≥35岁组的精子DFI异常率明显高于≤30岁组和30~35岁组(P=0.002)。在年龄≥35岁组中,年龄、PR%及精子总活力在DFI正常患者与DFI异常患者之间的差异具有统计学意义(P=0.006;P <0.001;P <0.001)。【结论】畸形精子症患者的PR%是与其精子DFI最密切相关的精子参数;年龄越大的畸形精子症患者的精子DNA完整性越差。【Objective】To study the correlation of sperm DNA integrity with age and routine semen parameters in ter-atozoospermic men.【Methods】Semen samples were collected from 255 teratozoospermic men between January 1,2009 and December 31,2016.Then we divided them into three groups according to the male age(≤30,30~35,≥35 years).The results of routine semen parameters were evaluated base on the WHO Laboratory Manual(5th ed).Sperm DNA integrity was detected by sperm chromatin dispersion(SCD)test.【Results】Sperm DNA fragmentation index(DFI)was inversely correlated with 2 parameters(progressive motility%and total motility%;P<0.001;P<0.001)and corre-lated with male age and NF%weakly(P=0.003;P=0.027)in teratozoospermic men.The correlation of sperm DFI with sperm concentration and total sperm number was not statistically significant(P=0.260;P=0.541).The patients in dif-ferent groups showed significant difference on the level of sperm DFI(P=0.021);the level of sperm DFI in≥35 years group was higher than≤30 years group and 30-35 years group(P=0.030,P=0.035);but there was no statistically significant difference of the sperm DFI level between≤30 years group and 30-35 year group(P=1.000).The abnormal-ity rate of sperm DFI in≥35 years group was higher than≤30 years group and 30-35 years group(P=0.002).The dif-ferences of age,PR%and total motility%are statistically significant between normal DFI patients and abnormal DFI pa?tients from≥35 years group(P=0.006;P<0.001;P<0.001).【Conclusion】The percentage of the progressive motili?ty is the sperm parameter which is most closely related to sperm DFI;The older teratozoospermic men have worse sperm DNA integrity than younger patients.

关 键 词：畸形精子症 精子DNA完整性 年龄 精液参数 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]