Au纳米簇-WS_2纳米片复合材料的合成及类过氧化物酶活性研究  

Synthesis of Au-WS_2 Nanocomposites and Study on Its Peroxidase Mimic Activity

作　　者：王文俊 吴燕虹 林秀玲 陈伟[1,2] 刘爱林 彭花萍[1,2] WANG Wen-Jun;WU Yan-Hong;LIN Xiu-Ling;CHEN Wei;LIU Ai-Lin;PENG Hua-Ping(Department of Pharmaceutical Analysis of the Fujian Medical University,Fuzhou 350004,China;The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province,Fujian Medical Univeristy,Fuzhou 350004,China)

机构地区：[1]福建医科大学药学院药物分析系,福州350108 [2]福建省高等学校纳米医药技术重点实验室,福州350108

出　　处：《分析化学》2018年第10期1545-1551,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21675024);福建省科技创新联合资金项目(Nos.2016Y9054;2016Y9056);福建省临床医学实验平台(附属第一医院)课题(No.FYKFKT-201707)资助~~

摘　　要：通过静电组装技术制备了金纳米簇-硫化钨片纳米复合材料(Au-WS_2NCs),此Au-WS_2NCs具有优良的过氧化物酶催化活性,能有效催化H_2O_2氧化3,3',5,5'-四甲基联苯胺盐酸盐(TMB)发生显色反应。考察了Au-WS_2催化NCs/TMB/H_2O_2显色反应条件对此纳米复合材料类过氧化物酶活性的影响,并进行了稳态动力学分析。实验结果表明,Au-WS_2NCs的酶催化反应遵循典型的Michaelis-Menten动力学模型。基于Au-WS_2NCs在H_2O_2存在下对TMB的催化显色反应及葡萄糖在葡萄糖氧化酶作用下可产生H_2O_2的催化氧化反应,在最优实验条件下,实现了比色法测定H_2O_2和葡萄糖,检出限分别为6.0×10^(-7)mol/L和3.1×10^(-6)mol/L,并成功用于血糖的检测。Gold nanoclusters-tungsten sulfide nanocomposites(Au-WS2 NCs)were synthesized through electrostatic self-assembly method.The synthesized Au-WS2 NCs could effectively catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)in the presence of H2O2 due to its excellent peroxidase-like catalytic activity.The effects of Au-WS2 NCs/TMB/H2O2 coloration conditions on peroxidase-like activity of Au-WS2 NCs were investigated and the steady-state kinetic analysis was carried out.The experimental results showed that the Au-WS2 NCs catalytic reaction using H2O2 and TMB as reaction substrates followed a typical Michaelis-Menten kinetic model.Based on the intrinsic peroxidase-like activity of Au-WS2 NCs and that glucose could be oxidized by glucose oxidase to produce H2O2,a colorimetric detection method for H2O2 and glucose was established and the detection limits were 6.0×10^-7 mol/L and 3.1×10^-6 mol/L,respectively.Thus,a new colorimetric method was developed,which provided a significant reference value for other types of peroxidase mimics.

关 键 词：金纳米簇 硫化钨纳米片 过氧化物模拟酶 比色分析法 

分 类 号：O657.3[理学—分析化学] TB33[理学—化学]