高强度间歇训练通过不同部位HSL磷酸化差异性激活减少内脏脂肪积累的机制研究  

High Intensity Interval Training Reducing Visceral Fat with Differential Activation of HSL Phosphorylation in Specific Site

作　　者：刘阳[1,2] 梅佳顺[1] 何玉秀 LIU Yang;MEI Jia-shun;HE Yu-xiu(Hebei Normal University,Shijiazhuang 050021,China;Hebei Normal University,Shijiazhuang 050021,China)

机构地区：[1]河北师范大学体育学院人体运动生物信息测评省重点实验室,河北石家庄050021 [2]河北师范大学体育学博士后科研流动站,河北石家庄050021

出　　处：《体育科学》2018年第9期56-64,共9页China Sport Science

基　　金：国家自然科学基金面上项目(31771319);河北省教育厅青年基金资助项目(QN2016186)

摘　　要：目的:探讨高强度间歇训练更显著减少内脏脂肪积累的可能机制,即激素敏感性脂肪酶(hormone-sensitive lipase,HSL)的磷酸化在不同部位(皮下脂肪与内脏脂肪)的差异性激活。方法:雌性c57bl/6小鼠进行高脂膳食饲养,建立饮食诱导的肥胖动物模型。将建模成功后的36只肥胖小鼠随机分为高脂膳食对照组(HFD组)、中等强度持续训练组(MICT组)和高强度间歇训练组(HIIT组)。HFD组继续进行12周高脂膳食饲养不运动,MICT组在高脂膳食饲养的同时进行约60%最大摄氧量的跑台持续运动,HIIT组在高脂膳食饲养的同时进行峰值强度约100%最大摄氧量的高强度间歇运动,并确保MICT组与HIIT组的奔跑距离相同。12周干预结束后测试小鼠体重、Lee’s指数,腹股沟(皮下)和子宫周围(内脏)脂肪重量;HE染色观察肝细胞脂肪侵润状态;比色法测试血脂4项(TC、TG、HDL-C、LDL-C);western测试内脏和皮下脂肪细胞HSL蛋白表达和ser563、ser565、ser660位点磷酸化。结果:以HFD组作为基线值比较,MICT组和HIIT组体重都出现了显著的下降(P<0.01),但二者对减少皮下脂肪积累无显著差异;HIIT相比MICT可以更显著的降低子宫周围(内脏)脂肪积累(P<0.01);HIIT组小鼠肝脏脂滴侵润程度低于HFD和MICT组,且能显著降低LDL-C水平(P<0.05);HIIT组小鼠子宫周围脂肪ser563磷酸化显著高于HFD和MICT组(P<0.05),MICT组腹股沟脂肪ser660显著高于HFD和HIIT组(P<0.05)。结论:高强度间歇训练相比中等强度持续训练,能更明显减少内脏脂肪积累,缓解肝脏脂肪病变,改善血脂异常,这与HIIT训练时内脏脂肪组织HSL-ser563磷酸化特异性提高,进而促进脂肪水解有关。Objective:To investigate the possible underlying mechanisms of why high-intensity interval training can significantly reduce the weight of visceral fat:differences in HSL(Hormone Sensitive Lipase)phosphorylation at different Sites(subcutaneous and visceral).Methods:Female c57bl/6 mice were fed a high-fat diet to produce a diet-induced obesity animal model.After successful modeling,36 obese mice were randomly divided into high-fat diet control group(HFD group),moderate-intensity continuous training group(MICT group)and high-intensity interval training group(HIIT group).The HFD group was fed a high-fat diet for 12 weeks without exercise.The MICT group continued to exercise on a treadmill of approximately 60%VO2max with high-fat diet,while the HIIT group was given a high-intensity interval training of approximately 100%VO2max peak intensity with high-fat diet.At the end of the 12 weeks training,mouse body weight,Lee’s index,inguinal(subcutaneous)and peri-uterine(visceral)fat weights were measured.Hepatocyte fat infiltration was observed by HE staining.Serum lipids(TC,TG,HDL-C,and LDL-C)were measured by colorimetry.HSL protein expression and phosphorylation of Ser563,ser565,ser660 were measured by Westernblot.Results:Compared with the HFD group as the baseline,there was a significant body weight decrease in the MICT group and HIIT group(P<0.01).HIIT and MICT showed no significant difference in subcutaneous fat reduction,but compared with MICT,HIIT could significantly reduce the peri-uterine(visceral)fat(P<0.01);Liver lipid droplet infiltration of HIIT group was lower than HFD and MICT,and LDL-C of HIIT significantly decreased(P<0.05);phosphorylation of ser563 in peri-uterine fat of HIIT was significantly higher than HFD and MICT(P<0.05).Ser660 of inguinal fat was significantly higher in MICT than in HFD HIIT groups(P<0.05).Conclusion:HIIT can reduce visceral fat,relieve hepatic fat lesions,and reduce LDL more than moderate-intensity continuous training.This is related to the fact that with HIIT training,the phospho

关 键 词：HIIT MICT HSL 皮下脂肪 内脏脂肪 

分 类 号：G804.7[文化科学—运动人体科学]