10月龄Wistar大鼠最大摄氧量测试方案的比较研究  

The Comparative Study on the Maximum Oxygen Uptake Test of 10-Month Old Wistar Rats

作　　者：覃飞[1,2] 董亚南 王松涛[3] 徐旻霄[1] 王中玮[1] 瞿超艺[1] 赵杰修 QIN Fei;DONG Ya-nan;WANG Song-tao;XU Ming-xiao;WANG Zhong-wei;QU Chao-yi;ZHAO Jie-xiu(China Institute of Sport Science,Beijing 100061 China;Jinan University,Guangzhou 510032 China;South China Normal University,Guangzhou 51006 China)

机构地区：[1]国家体育总局体育科学研究所,北京100061 [2]暨南大学体育学院,广东广州510032 [3]华南师范大学体育科学学院,广东广州510006

出　　处：《中国体育科技》2018年第5期65-69,共5页China Sport Science and Technology

基　　金：国家自然科学基金面上项目(11775059);中国博士后科学基金第61批面上项目(2017M610840);国家体育总局体育科学研究所基本科研业务费资助项目(基本17-18;基本17-19)

摘　　要：目的:最大摄氧量(VO_2max)是客观评价心肺功能的理想指标,也是制定运动强度的依据。在体育科学领域,实验动物常被用于探讨运动干预的效果和机制,因此,根据实验动物自身特征,制定个性化的VO_2max测试方案,保证VO_2max的准确性尤为重要。本研究选取Wistar大鼠为实验对象,设计不同的VO_2max测试方案并进行对比分析,以确定该年龄段大鼠的最佳VO_2max测试方案。方法:SPF级Wistar大鼠20只(雄性,10月龄),采用四通道大鼠代谢监测系统及大鼠运动跑台进行VO_2max测试。设计5种不同的VO_2max测试方案,每只大鼠按照随机顺序完成5种测试方案,各测试之间间隔休息3天。记录各方案测试过程中大鼠运动表现(配合程度、力竭状态)、摄氧量平台、方案完成时间、VO_2max和RER值等,根据运动表现及测试数据对比方案优劣。结果:1)共计12只大鼠完成全部5种测试方案,方案P1的VO_2max成功诱导率仅为58%,方案P2和P4为75%,方案P3和P5诱导率为83%;2)Bedford改良方案(P1)的运动递增负荷增幅过大,大鼠多呈现强烈应激,配合度明显下降,且有个别大鼠受伤或死亡,VO_2max成功诱导率低;3)方案P5诱导出的VO_2max值和RER值显著高于方案P1(P<0.05),方案P3诱导出VO_2max所需时间明显高于方案P1(P<0.01)和低于方案P5(P<0.05)。结论:对10月龄Wistar大鼠进行VO_2max的测试,在控制跑台适宜速度的情况下,以坡度变化作为主要的递增负荷实现方式,或优先考虑坡度的递增,可获得更佳的VO_2max测试结果,且大鼠的应激反应程度低,测试的依从性好,损伤和严重损害的风险低。Purpose:The maximum oxygen uptake(O2max)is an ideal index to objectively evaluate the cardiopulmonary function,as well as the basic to define exercise intensity.In the field of sports science,laboratory animals are often used to explore the effect and mechanism of exercise intervention.Therefore,it is very important to design optimal O2max test protocol and to ensure the accuracy of O2max according to the characteristics of the experimental animal itself.In this study,Wistar rats were selected,and various O2max test protocols were designed and analyzed to screen out the optimal O2max test protocol for the 10-month old wistar rats.Methods:20 SPF Wistar rats(male,10 month old)were tested for maximal oxygen uptake by a four channel metabolic monitoring system and running treadmill.Five different test protocols were executed.Each rat completed five test protocols in random order with 3 days’interval.The exercise performance(coordination degree,exhaustion state),oxygen uptake platform,finish time,O2max and RER value were recorded during the test of each program,and the performance and test data were compared.Results:1)12 rats completed all 5 test protocols of O2max.The induction ratio of O2max of P1 was only 58%,and P2 and P4 were 75%.While,the induction rate of P3 and P5 were both 83%.2)For the Bedford improvement protocol(P1),due to the intense increased exercise load,the rats showed more intense stress,the less coordination degree,injured even death,and lower induction rate of O2max.3)The O2max and RER values induced by the P5 are significantly higher than that of P1(P<0.05).The finish time of P3 is significantly higher than that of P1(P<0.01)and P5(P<0.05).Conclusion:For the O2max test for middle aged rats,with the suitable speed of the running treadmill,the change of gradient should be as the main way of increasing load,or increasing the gradient of the slope firstly,which can obtained optimal O2max,meanwhile reduce the stress response and the risk of injury and serious damage.

关 键 词：WISTAR大鼠 10月龄 最大摄氧量 呼吸商 测试方案 

分 类 号：G804.7[文化科学—运动人体科学]