全子宫切除术对BALB/c小鼠TDAR实验的影响  

Influence of total hysterectomy on TDAR in BALB/c mice

作　　者：常鹏环 黄守国[1] 林铃[1] 于欣荣 林莉香 杨照新 CHANG Peng-Huan;HUANG Shou-Guo;LIN Ling;YU Xin-Rong;LIN Li-Xiang;YANG Zhao-Xin(Department of Obstetrics and Gynecology of Haikou People′s Hospital,Haikou 570208,China)

机构地区：[1]海口市人民医院妇产科,海口570208 [2]海南医学院海南省药物临床前药理毒理学研究重点实验室,海口571199

出　　处：《中国免疫学杂志》2018年第9期1391-1393,1399,共4页Chinese Journal of Immunology

基　　金：本文受海南省卫生计生行业科研项目(No.18A200032)资助。

摘　　要：目的:通过建立BALB/c小鼠全子宫切除实验动物模型,考察和分析该术式对小鼠TDAR的影响。方法:平行对照组和手术组经腹行全子宫切除术,非手术组不予手术;非手术组和手术组腹腔注射KLH进行免疫;流式细胞技术分析B淋巴细胞亚群变化;ELISA法检测血清雌二醇和KLH Ig G抗体含量;HE染色观察卵巢组织学改变。结果:KLH能够刺激B淋巴细胞分化增殖,手术组和非手术组较平行对照组明显升高(P<0. 05);全子宫切除术能够影响KLH Ig G抗体生成,手术组较非手术组低(P<0. 05);全子宫切除小鼠雌二醇分泌明显低于非手术组(P<0. 05);全子宫切除小鼠卵巢组织出现体积缩小,成熟卵泡形态不规则,颗粒细胞松散、凋亡等。结论:全子宫切除术通过降低卵巢雌二醇分泌水平,对小鼠TDAR实验产生抑制效应。Objective:To investigate and analyze the influence of total hysterectomy model in BALB/c mice on TDAR.Methods:Mice were randomized to parallel control,operated and no-operated group.Parallel control and operated group underwent hysterectomy.The operated and no-operated group were immunized by intraperitoneal injection of KLH;The changes of Blymphocyte subsets were analyzed by flow cytometry.The concentration of estradiol and KLH IgG antibody in serum and the histological structure of the ovary were measured.Results:KLH could stimulate the proliferation and differentiation of B lymphocyte,and the operation group and no-operated group were significantly higher than those in the parallel control group(P<0.05).Total hysterectomy could affect the formation of KLH IgG antibody,and the operation group was lower than that of the no-operated group(P<0.05).The content of estradiol in the total hysterectomy mice was significantly lower than that in the no-operated group(P<0.05).Observation on ovarian histological structure of no-operated group was normal,however it were different for parallel control and operated group,such as the loss ovarian volume,the diseases of number of mature follicle,the loosing of granule cells,and apoptosis.Conclusion:Total hysterectomy has a inhibitory effect on TDAR test in mice by down-regulate the level of ovarian estradiol secretion.

关 键 词：子宫切除术 卵巢 TDAR 雌二醇 KLH 

分 类 号：R392.12[医药卫生—免疫学]