Anourosorexsquamipes染色体次缢痕多态性研究  

Heteromorphism of Secondary Constriction Regions of Anourosorex squamipes(Insectivora,Soricidae)

作　　者：李凤君 徐含峰 王旭明 韦海雪 张琪[1] 王琼[1] 陈顺德[1] LI Fengjun;XU Hanfeng;WANG Xuming;WEI Haixue;ZHANG Qi;WANG Qiong;CHEN Shunde(College of Life Science,Sichuan Normal University,Chengdu 610101,Sichuan;College of Life Science&Technology,Guangxi University,Nanning 530004,Guangxi)

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]广西大学生命科学与技术学院,广西南宁530004

出　　处：《四川师范大学学报（自然科学版）》2018年第6期813-816,共4页Journal of Sichuan Normal University（Natural Science）

基　　金：国家自然科学基金(31670388)、四川省教育厅重点基金(17ZA0324和17ZB0360)和成都市科技局项目(2015-NY02-00369-NC)

摘　　要：染色体的次缢痕区域与核仁组织区的形成有关.次缢痕的数量、位置和大小是染色体的重要形态特征.现有的研究报道了台湾短尾鼩的染色体次缢痕多态性,其近缘物种四川短尾鼩(Anourosorex squamipes)是否也存在这种次缢痕多态性还不清楚.采用骨髓细胞染色体标本制片法对成都地区的四川短尾鼩12个样本77个细胞进行染色体研究,结果表明:四川短尾鼩染色体组型为2n=48=2×16m+2×7sm+(X) m+(Y) sm,并发现四川短尾鼩的第21对和第22对常染色体上的次缢痕呈现数量多态性,其中10个细胞有1个次缢痕,50个细胞具有2个次缢痕,17个细胞具有3个次缢痕,这表明四川短尾鼩染色体次缢痕具有数量多态性.研究结果为进一步研究染色体次缢痕多态性发生的原因及其功能提供了基础资料.Secondary constriction is related to the formation of nucleolar organizing region.Its number,location and size are important morphological characteristics of chromosomes.Previous authors have reported considerable polymorphic status of the secondary constriction in the moleshrew Anourosorex yamashinai.However,whether there are heteromorphisms in Anourosorex squamipes is unknown.Twelve individuals of A.squamipes from Sichuan province were analyzed by marrow cell colchicinehypotonic airdrying technique for estimation of their Karyotype.77 cells were used to identify the number variation of secondary constriction.Our results indicated that the karyotype formula of A.squamipes was 2n=48=2×16m+2×7sm+(X)m+(Y)sm.Heteromorphism of secondary constriction in the autosomal pairs(No.21 and No.22)was firstly found.This study provides basic data for further exploration of the cause and function of this heterochromatin.

关 键 词：四川短尾闙 染色体 次缢痕 多态性 

分 类 号：Q958.1[生物学—动物学]