高山马铃薯种质资源遗传多样性的同工酶分析  

Genetic diversity analysis of alpine potato germplasm resources by isozyme

作　　者：洪森荣[1] 张铭心 叶思雨 宁本松 HONG Senrong;ZHANG Mingxin;YE Siyu;NING Bensong(College of Life Sciences,Shangrao Normal University,Shangrao 334001,China)

机构地区：[1]上饶师范学院生命科学学院,江西上饶334001

出　　处：《浙江农业学报》2018年第9期1445-1453,共9页Acta Agriculturae Zhejiangensis

基　　金：上饶师范学院2017年大学生创新创业训练计划(2017-CX-49)

摘　　要：对中国云南德宏和曲靖、湖北恩施、江西怀玉山高山马铃薯种质资源的过氧化物酶(POD)、酯酶(EST)和多酚氧化酶(PPO)同工酶进行了分析研究,以期揭示其遗传多样性。结果表明,云南德宏高山马铃薯、云南曲靖高山马铃薯和湖北恩施高山马铃薯均属于同一类,遗传分化程度较低,而怀玉山高山马铃薯单独成一类;用块茎和叶片做同工酶检测,会造成结果的差异,因而应保证取材的一致性。该实验结果可为进一步开展高山马铃薯品种遗传改良、杂交亲本选配以及高山马铃薯种质资源的利用提供依据。The genetic diversity of alpine potato germplasm resources in Yunnan Dehong,Yunnan Qujing,Hubei Enshi and Jiangxi Huaiyushan was analyzed by peroxidase(POD),esterase(EST)and polyphenol oxidase(PPO)isozyme in this paper.The results showed that Yunnan Dehong alpine potato,Yunnan Qujing alpine potato and Hubei Enshi alpine potato belonged to the same type,and the degree of their genetic differentiation was low,while the alpine potato in Jiangxi Huaiyushan was a separate group.Isozyme analysis of tubers and leaves would result in the difference of results,and isozyme analysis should ensure the consistency of sampling.The results of this study can provide the basis for further genetic improvement of potato varieties,selection of hybrid parents and utilization of alpine potato germplasm resources.

关 键 词：高山马铃薯 种质资源 遗传多样性 同工酶分析 

分 类 号：S532[农业科学—作物学]