农杆菌侵染条件下豇豆高频再生基因型的筛选  

Screening of cowpea genotypes with high-frequency regeneration in Agrobacterium-mediated transformation

作　　者：周雯[1,2] 吴新义[2] 汪宝根[2] 吴晓花[2] 鲁忠富[2] 汪颖 徐沛[2] 李国景[2] ZHOU Wen;WU Xinyi;WANG Baogen;WU Xiaohua;LU Zhongfu;WANG Ying;XU Pei;LI Guojing(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321000,China;Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321000 [2]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2018年第9期1489-1495,共7页Acta Agriculturae Zhejiangensis

基　　金："十三五"国家重点研发计划(2016YFD0100204-32);浙江省公益性项目(LGN18C150009);浙江省农业新品种选育重大科技专项"蔬菜新品种选育"领域(2016C02051-7-3);国家自然科学基金(31572135;31772299);浙江省自然科学基金(LY15C150002)

摘　　要：在农杆菌侵染条件下,对51份豇豆基因型材料进行组织培养和芽诱导分化试验。经初筛后,按再生率对不同基因型材料进行等级划分,对再生率在80%以上的10个基因型材料进行重复试验。结果表明,白八月豇的繁殖系数和不定芽诱导率均最高,分别为1.93和86.40%,该地方品种可在后续研究中作为一个较好的基因型材料用于转基因功能分析等研究。In this study,51 cowpea genotypes were tested for tissue culture and shoot induction in Agrobacterium-mediated transformation.After a preliminary screening for regeneration,the rate of shoot regeneration was graded and 10 genotypes with a regeneration rate above 80%were tested further in three replicates.White August Cowpea was found to show the highest reproduction coefficient and induction rate of adventitious buds,which were 1.93 and 86.40%,respectively.Therefore,this genotype may be used as a good explant source in the future transgenic studies.

关 键 词：豇豆 基因型 农杆菌 再生率 

分 类 号：S643.4[农业科学—蔬菜学]