以固态法一步合成的荧光碳点为探针测定人体尿液中阿霉素  

Synthesis of Fluorescent Carbon Dots via One-Step Solid-State Method and Their Application for Determination of Adriamycin in Urea Sample

作　　者：郭兴家[1] 张立志 王祚伟 刘文静 刘雪慧 刘清士 郝爱军[2] 李莹 GUO Xing-jia;ZHANG Li-zhi;WANG Zuo-wei;LIU Wen-jing;LIU Xue-hui;LIU Qing-shi;HAO Ai-jun;LI Ying(College of Chemistry,Liaoning University,Shenyang 110036,China;College of Pharmacy,Liaoning University,Shenyang 110036,China;Ji’nan Infectious Diseases Hosptial,Ji’nan 250021 ,China)

机构地区：[1]辽宁大学化学院,辽宁沈阳110036 [2]辽宁大学药学院,辽宁沈阳110036 [3]济南市传染病医院,山东济南250021

出　　处：《光谱学与光谱分析》2018年第10期3153-3158,共6页Spectroscopy and Spectral Analysis

基　　金：中国医药教育协会孙思邈中医药研究专项课题(2016SKT-M019);2016年辽宁省本科教改一般项目资助

摘　　要：以柠檬酸和尿素为碳源和氮源,采用固态法一步合成出量子产率高达23%的荧光碳点。表征结果表明,所合成的荧光碳点为平均粒径为3~4nm的球形,表面富含羟基、羧基和胺基等基团。此外,碳点的XRD谱图显示出无定型碳的特征峰。以所制备的碳点为荧光探针,基于碳点和阿霉素之间的共振能量转移而猝灭碳点的荧光,建立了阿霉素定量分析新方法。实验中考察了溶液的pH值和孵化时间的影响。在最佳实验条件下,阿霉素浓度在0.67~16.67μg·mL^(-1)范围之间与碳点的荧光猝灭值ΔF呈良好的线性关系(R2=0.995),检出限为0.22μg·mL^(-1),回收率为83.0%~89.2%,相对标准偏差小于2.5%(n=5)。尿样中常见物质对测定干扰较小,显示出所建立的方法具有较好的选择性。Fluorescent carbon dots(CDs)with a fluorescent quantum yield of 23%was successfully synthesized by one-step solid-state approach with citric acid and urea as carbon source and nitrogen source.It was found that the obtained CDs have highly disordered carbon structure and show sphere with an average diameter of 3~4 nm.In addition,the surfaces of CDs were covered with hydroxyl,carboxyl and amine groups.Based on the fluorescence resonance,energy transfers from fluorescence carbon dots to ADM(doxorubicin or adriamycin),the fluorescence of CDs can effectively quenched by ADM and a novel method for the sensitive and selective determination of ADM was proposed in this paper.Some experimental parameters,such as pH value of the reaction solution and reaction time were investigated.Under the optimum conditions,the decrease of CDs fluorescence intensity was in a good linearity with ADM in the concentration range of 0.67~16.67μg·mL-1(R 2=0.995),the detection limit of the method was found to be 0.22μg·mL-1,and the spiked recoveries ranged from 83.0%to 89.2%with RSD no more th an 2.5%.he result showed that common relevant substance,cations and anions did not significantly interfere with the detection of ADM.

关 键 词：荧光碳点 阿霉素 共振能量转移 荧光探针 

分 类 号：O433.5[机械工程—光学工程]