基于热红外成像的小麦条锈病早期检测（英文）  

Early Nondestructive Detection of Wheat Stripe Rust Using Infrared Thermal Imaging

作　　者：姚志凤[1,2] 何东健 雷雨[1,2] YAO Zhi-feng;HE Dong-jian;LEI Yu(College of Mechanical and Electronic Engineering,Northwest A&F University,Yangling712100,China;Key Laboratory of Agricultural Internet of Things,Ministry of Agriculture and Rual Affairs,Yangling712100,China)

机构地区：[1]西北农林科技大学机械与电子工程学院,陕西杨凌712100 [2]农业部农业物联网重点实验室,陕西杨凌712100

出　　处：《光谱学与光谱分析》2018年第10期3303-3309,共7页Spectroscopy and Spectral Analysis

基　　金：Key Science and Technology Program of Shaanxi Province(2015KTZDNY01-06,2017NY-104);National Natural Science Foundation of China(31701326);Science and Technology Plan Project of Yangling Demonstration Area(2015NY-10)

摘　　要：由条形柄锈菌引起的小麦条锈病是一种世界性范围内普遍流行的重大病害,实现其早期检测对病害的有效防控与小麦的安全生产具有重要意义。旨在实验室环境下分析小麦接种条锈病菌后16天的热红外图像与叶片温度变化,研究热红外成像技术用于小麦条锈病早期检测的可行性。实验发现,随着接种天数的增加,接种病害的小麦植株较健康小麦,叶片的平均温度会逐渐降低,叶片间的最大温差会不断加大。接种后第6天,热红外成像技术可观测到小麦条锈病病斑,较肉眼观察提前了4天。接种后第16天,接种叶片的平均温度比健康叶片低2.5℃,最大温差比健康叶片高2.28℃。同时,通过对健康、发病以及潜伏期的小麦叶片的显微结构分析,发现条锈病孢子入侵叶片,使细胞结构破环,气孔导度增大,蒸腾作用加剧,从而导致叶片表面温度降低。可见,热红外成像技术可以对小麦条锈病进行早期检测。Wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,is one ofthemost destructive diseases causing severe decreases in wheat yield.Early detection of wheat stripe rust before symptom appearance is of great significance for developing effective control strategies and taking timely management measures toensure high and stable yield of wheat.The objective of this study is to investigate the possibility of thermal infrared imaging technology used for early detection of wheat stripe rust by assessing the temperature changes of normal and infected wheat leaves in a pot experiment under controlled conditions.Four pots ofwheat plants were subjected to artificial inoculation with Puccinia striiformis once a day,lasting for 16 days,in an artificial climate chamber.Meanwhile,healthy wheat plants were chosen as the normal control.Thermal infrared images and data on leaf temperature of all the normal and infected wheat leaves were collected 16 days after inoculation(dpi)by using an infrared thermography.The results revealed that with the increasing of days after inoculation,the divergence in the average temperature and maximum temperature difference(MTD)between infected and healthy wheat leaves gradually increases.The infected wheat leaves can be distinguished from healthy ones 6 days after inoculation using infrared thermal imaging,that is,at least 4 days before visible symptoms appearance.At 16 dpi,the average temperature of the inoculated wheat leaves was 2.5℃lowerthan that of healthy ones,and the MTD of the inoculated leaves was 2.28℃larger than that of healthy ones.Fungal development was also assessed microscopically.It was found through microscopic observation and analysis that stripe rust(Puccinia striiformis)infection caused changes in the integrity of the epidermal cells,the structure of chloroplasts,as well as stomatal conductance and leaf transpiration rate involved in inducing defense.A decrease in leaf surface temperature after the infection was observed as a thermal signature of early infection of d

关 键 词：热红外成像 小麦条锈病 叶片温度 早期检测 潜伏期 显微成像 

分 类 号：S431.9[农业科学—农业昆虫与害虫防治]