蛇床子素对大鼠颈动脉内膜增生的影响  

Effect of Osthole on carotid intimal hyperplasia in rats

作　　者：张宇[1] 孙淑娴[1] 马威[1] 李昌义[1] 王斌[1] 王兆祥[1] 亢小丽[1] 纪征[1] Yu Zhang;Shu-xian Sun;Wei Ma;Chang-yi Li;Bin Wang;Zhao-xiang Wang;Xiao-li Kang;Zheng Ji(Tangshan Gongren Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]河北省唐山工人医院心内一科,河北唐山063000

出　　处：《中国现代医学杂志》2018年第23期21-25,共5页China Journal of Modern Medicine

摘　　要：目的研究蛇床子素对球囊损伤后大鼠颈动脉内膜增生的影响。方法 SD雄性大鼠30只,随机分为假手术组、模型组、低剂量蛇床子素组[10 mg/(kg·d)]和高剂量蛇床子素组[30 mg/(kg·d)]。复制大鼠颈动脉球囊损伤模型,蛇床子素组大鼠术前2 d开始给药,假手术组、模型组同时给予0.9%Na Cl,共给药16 d。术后第13天行苏木精-伊红染色法染色,观察血管内膜的形态学变化;采用免疫荧光法检测新生内膜增殖细胞核抗原(PCNA)的表达,ELISA检测大鼠受损血管中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)水平,Western blot检测核转录因子κB(NF-κB)和Toll样受体(TLR4)蛋白的表达。结果与模型组相比,高、低剂量蛇床子素组均能减少球囊损伤后新生内膜面积、内膜/中膜比(P<0.05),并使PCNA阳性指数降低(P<0.05),降低球囊损伤血管组织TLR4和NF-κB的过表达和炎症介质TNF-α和IL-1β水平(P<0.05)。结论蛇床子素能够抑制大鼠颈动脉球囊损伤后的内膜增生,主要通过影响TLR4/NF-κB通路,减轻炎症反应,抑制血管平滑肌细胞的增殖而发挥作用。Objective To investigated the effect of Osthole on carotid intimal hyperplasia in rats after balloon injury.Methods Male SD rats were randomly assigned to a sham group,a model group,a low-dose group and a high-dose group.The rats of the model group were injured by balloon catheter.The rats in the low-dose group and the high-dose group were given 10 mg/(kg·d)and 30 mg/(kg·d)respectively for 16 days while those in the sham group and model group were given normal saline.On the 13th day after balloon injury,HE staining was applied for observation of the morphological changes of carotid intima,immunohistochemical staining was used to detect the expression of proliferating cell nuclear antigen(PCNA)in neointima,ELISA was employed to determine the levels of tumor necrosis factorα(TNF-α)and IL-1β,and Western blot was performed to test the expressions of nuclear factor kappa B(NF-κB)and Toll-like receptor(TLR4)proteins.Results Compared with the model group,Osthole significantly decreased the neointima area and the intimal/media ratio,markedly reduced positive index of PCNA expression.Furthermore,Osthole significantly inhibited NF-κB and TLR4 over-expressions induced by balloon injury and reduced the levels of TNF-αand IL-1β.Conclusions These findings suggest that Osthole is involved in the control of neointimal formation via inhibiting TLR4 and NF-κB activation,decreasing inflammatory reaction and inhibiting the proliferation of vascular smooth muscle cells.

关 键 词：蛇床子素 球囊损伤 血管平滑肌 内膜增生 

分 类 号：R285.5[医药卫生—中药学]