用于aPTT测量的Parylene-C增强型石英晶体微天平耗散检测系统  

Parylene-C enhanced quartz crystal microbalance based dissipation detecting system for aPTT measurement

作　　者：丰斌 姚佳[2,3] 张威 孔慧[2] 魏巍[2] 周连群 FENG Bin;YAO Jia;ZHANG Wei;KONG Hui;WEI Wei;ZHOU Lian-qun(Shanghai University,School of Communication&Information Engineering,Shanghai 200444,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China;Soochow University,School of Electronic and Information Engineering,Suzhou 215006,China)

机构地区：[1]上海大学通信与信息工程学院,上海200444 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215163 [3]苏州大学电子信息学院,江苏苏州215006

出　　处：《光学精密工程》2018年第9期2304-2311,共8页Optics and Precision Engineering

基　　金：国家重点研发计划资助项目(No.2018YFC0115705);国家自然科学基金资助项目(No.51675517);江苏省优秀青年基金资助项目(No.BK20160057);中国科学院青年创新促进会资助(No.2014280;No.2018360);中国科学院科研装备研制项目资助(No.YZ201638);江苏省重大产业项目资助(No.BO2015007);苏州市民生科技项目资助(No.S201749)

摘　　要：房颤、血栓等患者抗凝药物日常口服等场景迫切需要凝血参数快速检测,本文设计和制作了一种Parylene-C增强型石英晶体微天平(QCM)传感器及其耗散因子检测系统用于凝血测量。首先使用Parylene-C有效增加石英晶体微天平传感器的峰峰值和有效使用次数,基于传感器耗散因子对血液凝固过程血液粘弹性变化敏感,设计电导谱分析法的压电传感器耗散因子快速测量系统,对血浆部分凝血活酶时间(aPTT)进行测量。并用SYSMEX CS 5100光学凝血仪、Lambda 950分光光度计验证系统测量结果。实验表明,Parylene-C增强型QCM传感器信号峰峰值增加8±1%,传感器aPTT实验有效重复使用次数为30次,系统30℃温差最大耗散偏移2.09×10-6。aPTT耗散曲线与光学法(lambda950)吸光度曲线变化趋势一致。与SYSMEX CS 5100临床结果线性拟合决定系数R2为0.99。同样本10次重复实验结果变异系数为1.48%。Parylene-C增强型QCM传感器与耗散分析法的联合应用具备多场景下凝血参数快速检测的能力,系统温度稳定性好,具有满足即时检测应用的潜力。To address the need to rapidly detect blood coagulation in daily administration of anticoagulant for atrial fibrillation and thrombus patients and others,a Parylene-C enhanced quartz crystal microbalance(QCM)sensor and a dissipation detection system were designed and manufactured for the measurement of coagulation parameters.Parylene-C was used to enhance the peak-to-peak value and reusability of the QCM sensor.A dissipation detection system for piezoelectric sensors was designed using the principle of conductance spectrum analysis to measure the activated partial thromboplastin time(aPTT)based on the sensitivity of the sensor s dissipation to the change of blood viscoelasticity in the coagulation process.The SYSMEX CS 5100 optical coagulometer system and Lambda 950 spectrophotometer were used to evaluate the system.The results indicate that Parylene-C increases the sensor s peak-to-peak value by 8±1%and it can be reused 30 times.The system has a maximum dissipation excursion of 2.09×10-6 with a temperature difference of 30℃.The dissipation and absorbance curves of optical detection(Lambda 950)have the same variation trend.The adjusted R-square value of the linear fitting with SYSMEX CS 5100 is 0.99.The results of the experiment,repeated 10 times with the same sample,have a variable coefficient of 1.48%.The combination of the Parylene-C enhanced QCM and dissipation detection system is highly temperature-stable,can rapidly detect blood coagulation in different scenarios,and has the potential for point-of-care testing.

关 键 词：Parylene-C 石英晶体微天平 耗散因子 凝血功能检测 部分凝血活酶时间(aPTT) 

分 类 号：TP394.1[自动化与计算机技术—计算机应用技术] TH691.9[自动化与计算机技术—计算机科学与技术]