中药复方多糖对不同MHC B-Lβ Ⅱ基因型蛋鸡免疫活性的影响  

Effects of Traditional Chinese Medicinal Compound Polysaccharides on Immune Activity in Different MHCB LβⅡ Genotype Laying Hens

作　　者：刘晓婷[1] 朱晓庆[1] 杨莉[1] 马昭[1] 乔彦杰 张保军 谷新利[1] 商云霞[1] LIU Xiaoting;ZHU Xiaoqing;YANG Li;MA Zhao;QIAO Yanjie;ZHANG Baojun;GU Xinli;SHANG Yunxia(College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《西北农业学报》2018年第9期1265-1272,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31460673)。

摘　　要：探究肌注中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡血清中细胞因子质量浓度(IL-1β、IL-10、IL-15)与IBDV抗体效价的影响规律。采用PCR-SSCP方法对试验鸡按不同基因型进行分组,将各组分成高、中、低剂量的多糖试验组和空白对照组。各组鸡分别于8日龄时肌肉注射0.2 mL的50 mg/mL、25mg/mL、12.5mg/mL中药复方多糖和生理盐水,连续7d,于21、27、35、42和49日龄时从各组随机抽取5只鸡,翅下静脉采血并分离血清,利用ELISA法检测各试验组和空白组21、27、35、42和49日龄血清中细胞因子的质量浓度及IBDV抗体效价。结果显示,中药复方多糖组血清中IL-1β和IL-15的质量浓度及IBDV抗体效价均显著高于对照组,而IL-10的质量浓度则显著低于对照组,且不同剂量多糖组和基因型间存在差异。其中,AA基因型鸡中剂量多糖组的血清中IL-1β和IL-15的质量浓度及IBDV抗体效价显著高于高、低剂量组,IL-10显著低于高、低剂量组;BB基因型鸡高剂量多糖组的血清中IL-1β和IL-15的质量浓度及IBDV抗体效价显著高于中、低剂量组,IL-10显著低于中、低剂量组;BC基因型鸡中各剂量多糖组间血清中IL-1β、IL-15和IL-10的质量浓度及IBDV抗体效价并无显著差异。结果表明,不同MHC B-LβII基因型鸡个体之间存在免疫应答能力的差异,不同剂量中药复方多糖对调节鸡免疫活性的最适基因型不同。To investigate the effects of intramuscular injection of traditional Chinese medicinal compound polysaccharides on serum cytokines(IL-1β,IL-10,IL-15)and IBDV antibody titer in different MHC B-LβII genotypes of laying hens.PCR-SSCP technique was applied to group the experimental hens according to different MHC B-LβII genotypes,the hens were divided into four groups.Hens in each group at eight-day-age were intramuscularly injected with 0.2 mL traditional Chinese medicinal compound polysaccharides with the concentration of 50 mg/mL(high dose),25 mg/mL(medium dose),12.5 mg/mL(low dose)and physiological saline(control),continuously for 7 days.At 21-,27-,35-,42-and 49-day-old of ages,five chicken blood samples in each group were collected,and sera were separated for detection of cytokine contents and IBDV antibody titers.The results showed that,the mass concentrations of IL-1β,IL-15 and the titer of IBDV in the serum of compound polysaccharide groups were significantly higher than those in the control group,while the mass concentration of IL-10 was significantly lower than that of the control group,and there were differences between different doses of polysaccharides and genotypes.Among them,the mass concentrations of IL-1β,IL-15 and the titer of IBDV in serum of AA genotype chickens were significantly higher than those of high and low dose groups,while IL-10 was significantly lower than those of high and low dose groups.The mass concentrations of IL-1β,IL-15 and IBDV antibody in serum of BB genotype chickens of high dose polysaccharide group were significantly higher than those of middle and low dose group,while IL-10 was significantly lower than those of middle and low dose group.There were no significant differences between the concentration of IL-1β,IL-15,IL-10 and IBDV antibody in serum of BC genotype chickens.The results showed that there were differences in immune response between individuals with different MHC B-LβII genotypes chicken.Different doses of traditional Chinese medicine compound polysacchari

关 键 词：中药复方多糖 MHC B-LβII基因 细胞因子 抗体效价 免疫活性 

分 类 号：S853.72[农业科学—临床兽医学]