甘草内生细菌对4种植物病原菌的抑菌谱及其16SrRNA系统发育分析  

Antimicrobial Spectrum of Endophytic Bacteria from Glycyrrhiza spp. Against Four Phytopathogens and Its Phylogenetic Analysis Based on 16S rRNA

作　　者：祖丽皮亚木·木沙尔 李丽[2] 郭建伟[2,3] 古丽斯玛依·艾拜都拉[1] OSAMA Mohamed[2,4] ZULPIYA·Musa;LI Li;GUO Jianwei;GULSUMAY·Abaydulla;OSAMA Mohamed(Xinjiang Laboratory of Resources Microbiology,College of Life Sciences and Technology,Xinjiang University, Urumqi 830046,China;Key Laboratory of Biogeography and Bioresource in Arid Land,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;Key Laboratory of Higher Quality and Efficient Cultivation and Security Control of Crops for Yunnan Province,Honghe University,Mengzi Yunnan 661100,China;Environmental Science Department,Institute for Postgraduate Environmental Studies,Suez Canal University,El-Arish Branch,North Sinai 45511,Egypt)

机构地区：[1]新疆大学生命科学与技术学院新疆资源微生物实验室,乌鲁木齐830046 [2]中国科学院新疆生态与地理研究所干旱区生物地理与生物资源重点实验室,乌鲁木齐830011 [3]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [4]Environmental Science Department,Institute for Postgraduate Environmental Studies,Suez Canal University,El-Arish Branch,Nor

出　　处：《西北农业学报》2018年第9期1367-1374,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：中国科学院西部之光项目(XBBS201305);国家自然科学基金青年项目(No.31200008)和地区项目(31860026);红河学院第二批中青年学术骨干培养对象(2015GG0206);红河学院博硕启动项目(XJ15B16);红河学院应用型研究项目(XJY15Y02)。

摘　　要：旨在筛选对4种植物病原真菌具有拮抗活性的甘草内生细菌,为新疆甘草内生细菌的开发应用提供理论依据。采用平板对峙培养法从100株甘草内生细菌中筛选对石榴枯萎病甘薯长喙壳、草果叶斑病镰刀菌、草果假茎黑斑病小孢拟盘多毛孢、墨兰炭疽病胶孢炭疽菌等4个病原菌具有拮抗活性的菌株,并测定其16SrRNA基因序列,建立系统发育树对其进行初步鉴定。结果显示,61株菌株对甘薯长喙壳具有拮抗作用,其中13株菌的抑菌半径均大于15mm。进一步研究表明,10株菌对4种植物病原菌都表现出较高的抑菌能力。基于16Sr RNA基因序列分析与系统发育分析表明,其中11株菌为萎缩芽孢杆菌(Bacillus atrophaeus),另外2株菌分别为副氧化微杆菌(Microbacterium paraoxydans)和莫哈韦芽胞杆菌(B.mojavensis)。In order to provide a theoretical basis for the exploration and utilization of endophytic bacteria of Glycyrrhiza,it is our aim to screen antagonistic strains of endophytic bacteria isolated from Glycyrrhiza against 4 kinds of plant pathogens.A total of 100 endophytic bacterias recovered from Glycyrrhiza were studied for its antagonism against Ceratocystis fimbriata,Pestalotiopsis microspora,Fusarium sp and Colletotrichum gloeosporioides Allesch by dual culture method.A phylogenetic tree were constructed based on its 16S rRNA gene sequences of the strains.The results indicated that 61 strains had inhibition activity to pomegranate wilt pathogen in vitro condition,and 13 strains showed strong antagonistic activity with an inhibition zone(>15 mm).The further study showed that 10 strains were high antagonistic activity against 4 kinds of plant pathogens.The identification result based on 16S rRNA gene sequence and phylogenetic tree demonstrated that 11 strains belonged to Bacillus atrophaeus and the other two strains belonged to Microbacterium paraoxydans and Bacillus mojavensis.

关 键 词：甘草内生细菌 植物病原菌 拮抗作用 系统发育 

分 类 号：Q939.95[生物学—微生物学] S567.71[农业科学—中草药栽培]