芦笋总皂苷的提取纯化及抗氧化研究  

Study on Extraction Purification and Antioxidant Activity of Total Saponins from Asparagus officinalis L.

作　　者：葛思琪 赵庆生[2,3] 孙广利 赵兵 陈哲[1,2] 黄云祥 查圣华 GE Si-qi;ZHAO Qing-sheng;SUN Guang-li;ZHAO Bing;CHEN Zhe;HUANG Yun-xiang;ZHA Sheng-hua(Beijing City University,Beijing 100094,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100090,China;Hebei Asparagus Industry Technology Research Institute,Hebei Asparagus Engineering Research Center,Qinhuangdao 066000,Hebei,China)

机构地区：[1]北京城市学院,北京100094 [2]中国科学院过程工程研究所,北京100090 [3]河北省芦笋产业技术研究院,河北省芦笋工程技术研究中心,河北秦皇岛066000

出　　处：《食品研究与开发》2018年第20期57-62,共6页Food Research and Development

摘　　要：以芦笋总皂苷的提取率为考察指标,香草醛-冰醋酸法测定芦笋总皂苷的含量,采用正交设计法,对乙醇浓度、提取温度、提取时间、料液比4个因素进行考察,优化芦笋总皂苷提取工艺参数。芦笋总皂苷的最优工艺为:乙醇浓度为90%,提取温度为80℃,料液比为1∶40(g/mL),提取时间为1 h。在此条件下芦笋总皂苷提取率为10.68%。利用HPD-100大孔树脂对芦笋总皂苷提取液进行纯化,分别使用水、20%、50%、80%、95%的乙醇进行梯度洗脱。结果表明在乙醇浓度为50%的第3个流分时,总皂苷含量最高为51.0%。抗氧化试验表明,芦笋总皂苷纯化前后清除DPPH自由基的IC_(50)分别为147.43、140.05μg/mL;清除ABTS+自由基的IC_(50)分别为212.76、169.70μg/mL。The extration process of total saponins from asparagus was determined by vanillin-glacial acetic acid method,the four factors including ethanol concentration,extraction temperature,extraction time and material liquid ratio were studied via orthogonal design and optimized total saponins extraction process parameters were acquired as follows:ethanol concentration was 90%,extraction temperature was 80℃,material liquid ratio was 1∶40(g/mL),and extraction time was 1 h.Under this condition,the total saponins extraction rate was 10.68%.The macroporous resin was used to remove impurities from the total saponins extract of asparagus and gradient elution was performed using pure water,20%,50%,80%,and 95%ethanol.The results showed that the highest total saponin content was 51.0%at the third fraction with an ethanol concentration of 50%.The antioxidant activities of purified extracts measured by DPPH·and ABTS+·radical scavenging activity assay,the IC50 for DPPH radicals was 147.43μg/mL and 140.05μg/mL;the IC50 for ABTS+radicals were 212.76μg/mL and 169.70μg/mL.

关 键 词：芦笋 总皂苷 提取 纯化 抗氧化 

分 类 号：TQ464.3[化学工程—制药化工]