基于高通量测序的不同填闲作物对黄瓜连作土壤微生物群落结构的影响  

Effects of Different Cover Crops on Soil Microbial Community Structure in Cucumber Continuous Cropping Based on High-throughput Sequencing

作　　者：田晴 李敏 吴凤芝 TIAN Qing;LI Min;WU Feng-zhi(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2018年第10期60-68,共9页China Vegetables

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23-B10)

摘　　要：以连作3 a黄瓜的日光温室土壤为研究对象,以黄瓜为主栽作物,分别以大葱、普通白菜和小麦为冬夏季休闲期的填闲作物,采用高通量测序技术分析不同填闲模式对黄瓜连作土壤微生物群落结构的影响。结果表明:3种填闲模式处理的土壤细菌群落和真菌群落中的主要物种基本一致,但是相对丰度存在差异。在细菌属水平上,填闲小麦处理中Flexibacter、Nitrosomonadaceae_uncultured、Anaerolineaceae_unculture的相对丰度显著低于填闲大葱和普通白菜,而Flavobacterium、Clostridium、Peptostreptococcaceae_incertae_sedis的相对丰度显著高于填闲大葱和普通白菜;在真菌属水平上,填闲小麦处理中Ascomycota_unclassified、Hypocreales_unclassified、Humicola的相对丰度显著低于填闲大葱和普通白菜,而Fusarium、Pyrenochaeta、Nectriaceae_unclassified、Preussia的相对丰度显著高于填闲大葱和普通白菜。说明不同填闲作物对土壤微生物群落结构和多样性的影响存在差异。Taking the solar greenhouse soil for 3 years successive cucumber cropping as research object,this paper analyzed the effects of different filling patterns on soil microbial community structure by high-throughput sequencing technology under successive cucumber cropping.In this study,cucumber was the main crop,scallion,cole and wheat were the crops for winter and summer leisure period.The results indicated that the major species in soil bacterial community and fungus community by 3 different filling patterns basically had no difference,but there were differences in relative abundances among them.At the level of bacteria,among filling wheat treatment,the relative abundances of Flexibacter,Nitrosomonadaceae_uncultured,naerolineaceae_unculture were significantly lower than those of scallion and cole.While,the relative abundances of Flavobacterium,Clostridium and Peptostreptococcaceae_incertae_sedis were significantly higher than those of scallion and cole.At the level of fungus,among filling wheat treatment,the relative abundances of Ascomycota_unclassified,Hypocreales_unclassified,Humicola were significantly lower than those of scallion and cole.While,the relative abundances of Fusarium,Pyrenochaeta,Nectriaceae_unclassified and Preussi were significantly higher than those of scallion and cole.These results indicated that there were differences in the effects of different cover crops on soil microbial community structure and diversity.

关 键 词：黄瓜 连作土壤 填闲作物 高通量测序 真菌群落 细菌群落 

分 类 号：S642.2[农业科学—蔬菜学] S154.3[农业科学—园艺学]