乌梅的~1H-NMR指纹图谱研究  

Study on ~1H-NMR Fingerprints of Prunus mume

作　　者：唐荣伟[1] 田玫瑰 唐玲[1] 杜欢 范刚[2] 潘正[3] TANG Rongwei;TIAN Meigui;TANG Ling;DU Huan;FAN Gang;PAN Zheng(Dept.of Clinical Medicine,Dazhou Vocational and Technical College,Sichuan Dazhou 635001,China;College of Ethnic Medicine,Chengdu University of TCM,Chengdu 611137,China;College of TCM,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]达州职业技术学院临床医学系,四川达州635001 [2]成都中医药大学民族医药学院,成都611137 [3]重庆医科大学中医药学院,重庆400016

出　　处：《中国药房》2018年第19期2644-2647,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81303310)

摘　　要：目的:为乌梅的质量控制以及功效物质的全面检测和开发利用提供参考。方法:以氘代甲醇-重水(1∶0.3,V/V)为提取溶剂,在25℃下采用标准的预饱和脉冲序列(ZGPR)压制水峰建立10批不同来源乌梅药材的氢核磁共振(~1H-NMR)指纹图谱,通过"加标准品定性"试验、数据库比对和相关文献比对进行信号归属,采用相关系数法计算10批不同来源乌梅药材~1H-NMR指纹图谱的相似度,并采用主成分分析(PCA)法进行药材的质量评价。结果:从乌梅药材中同时检测出19种化合物,包括绿原酸、柠檬酸、丁二酸、乙酸、丙氨酸、缬氨酸、γ-氨基丁酸等。其中,乙酸、γ-氨基丁酸和丁二酸为首次从乌梅药材中检测到。10批乌梅药材指纹图谱的相似度均>0.9,平均为0.974。PCA结果显示,前两个主成分的累积贡献率为90.3%,与相似度评价结果基本一致。结论:建立的~1H-NMR方法具有整体性和分析快等优点,可为乌梅的质量控制和功效物质的全面检测提供参考。OBJECTIVE:To provide reference for the quality control of Prunus mume,comprehensive detection,development and utilization of active ingredients.METHODS:Using deuterated methanol and deuterium oxide(1∶0.3,V/V)as extracting solvent,1H-NMR fingerprints of 10 batches of P.mume from different sources were established by standard presaturated pulse sequence(ZGPR)at 25℃.The signal was assigned through“adding standard substance qualitation”trial,database comparison and related literature comparison.The similarity of 1H-NMR fingerprints was calculated for 10 batches of P.mume from different sources by using correlation coefficient method.The quality of medicinal material was evaluated by principal component analysis(PCA).RESULTS:A total of 19 compounds were detected in P.mume,among which chlorogenic acid,citric acid,succinic acid,acetic acid,alanine,valine,γ-aminobutyric acid,etc.Among them,acetic acid,γ-aminobutyric acid and succinic acid were detected for the first time.Moreover,the similarity of 1H-NMR fingerprinting of 10 samples was higher than 0.9,being 0.974 in average.The results of PCA showed that accumulative contribution rate of former 2 main components was 90.3%,which was consistent with the similarity evaluation.CONCLUSIONS:The established 1H-NMR method has the advantages of embodying integrity and rapid analysis,and can provide reference for the quality control of P.mume,and comprehensive testing of active ingredients.

关 键 词：乌梅 氢核磁共振 指纹图谱 主成分分析 质量控制 

分 类 号：R917[医药卫生—药物分析学]