药效学结合正交试验优选蛇归止痒方的提取工艺  

Optimization of the Extraction Technology of Shegui Zhiyang Formula by Pharmacodynamics Combined with Orthogonal Test

作　　者：张周菊[1] 张悦 彭翠香 孔菲菲[1] 高佩 梁璐[1] ZHANG Zhouju;ZHANG Yue;PENG Cuixiang;KONG Feifei;GAO Pei;LIANG Lu(Hubei Pharmaceutical Industry Research Institute Co.,Ltd.,Wuhan 430065,China)

机构地区：[1]湖北省医药工业研究院有限公司,武汉430065

出　　处：《中国药房》2018年第19期2662-2666,共5页China Pharmacy

基　　金：湖北省科技发展专项项目(No.2016-205-009-001)

摘　　要：目的:优化蛇归止痒方提取工艺。方法:以小鼠止痒药效实验相关结果为指标筛选蛇归止痒方的提取工艺路线(包括醇提、水提等4种工艺),再以收膏率及阿魏酸、芍药苷、苦参碱、氧化苦参碱含量等组成的综合评分为考察指标,采用L9(34)正交试验筛选乙醇体积分数、加醇量倍数、提取时间(次数)等提取工艺的最优参数,并进行工艺验证试验。结果:筛选的提取工艺路线为全方采用60%乙醇回流提取;最优工艺中各参数具体为提取2次,第1次加8倍乙醇提取2 h,第2次加8倍乙醇提取1 h。验证试验结果显示,3次试验阿魏酸、芍药苷、苦参碱、氧化苦参碱的平均含量分别为0.71、22.13、1.92、9.88 mg/g(RSD=2.96%、2.03%、2.55%、1.23%,n=3),平均收膏率为19.22%(RSD=0.96%,n=3)。结论:优选的提取工艺稳定、可行。OBJECTIVE:To optimize the extraction technology of Shegui zhiyang formula.METHODS:The extraction technology route(4 technologies such as ethanol extraction,water extraction,etc.)of Shegui zhiyang formula in mice was screened by using related indexes of antipruritic efficacy test.Using comprehensive score of extract yield,the contents of ferulic acid,paeoniflorin,matrine and oxymatrine as indexes,L9(34)orthogonal test was used to screen optimal parameters of extraction technology as volume fraction of ethanol,ethanol fold,extraction time(times).Validation test was conducted.RESULTS:Screened extraction technology route was medicinal materials were reflux extracted with 60%ethanol.The optimal technology included extracting for 2 times,lasting for 2 h with 8-fold ethanol at first time,lasting for 1 h with 8-fold ethanol at second time.The results of validation tests showed that average contents of ferulic acid,paeoniflorin,matrine and oxymatrine were 0.71,22.13,1.92,9.88 mg/g(RSD=2.96%,2.03%,2.55%,1.23%,n=3).Average extract yield was 19.22%(RSD=0.96%,n=3).CONCLUSIONS:The optimized extraction process is stable and feasible.

关 键 词：蛇归止痒方 提取工艺 正交试验 优化 止痒药效实验 小鼠 

分 类 号：R285.5[医药卫生—中药学] R283[医药卫生—中医学]