5个马铃薯新品系染色体构型及遗传差异的SSR分析  

SSR Analysis of Chromosome Pairing Configuration and Genetic Differences of 5 New Strains of Potato

作　　者：于卓[1] 李景伟 于肖夏[1] 苏娜[1] 张明飞[1] 马艳红[1] 杨东升[1] YU Zhuo;LI Jingwei;YU Xiaoxia;SU Na;ZHANG Mingfei;MA Yanhong;YANG Dongsheng(Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China)

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《种子》2018年第9期18-21,27,共5页Seed

基　　金：内蒙古科技计划重点项目(2016ZD);内蒙古农业大学科技成果转化专项资金动植物品种培育项目(YZGC 2017006);内蒙古自治区高等学校青年科技英才支持计划(NJYT-18-B 04)

摘　　要：为明确自3个杂交组合F1代分离群体中选育出的5个马铃薯优良新品系NNS-C 2、NNS-A 6、NNS-A 10、NNS-B 2和NNS-A 11的分子细胞遗传学差异,本试验以亲本为对照,采用常规压片法和SSR分子标记技术对5个新品系进行了染色体配对构型和SSR分析。研究表明,5个新品系NNS-C 2、NNS-A 6、NNS-A 10、NNS-B 2和NNS-A 11的染色体配对构型有一定差异,依次分别为1.95Ⅰ+15.46Ⅱ+1.35Ⅲ+2.77Ⅳ、5.66Ⅰ+11.47Ⅱ+3.6Ⅲ+2.15Ⅳ、2.98Ⅰ+13.54Ⅱ+2.2Ⅲ+2.83Ⅳ、8.27Ⅰ+9.26Ⅱ+4.43Ⅲ+1.98Ⅳ和3.47Ⅰ+12.13Ⅱ+3.21Ⅲ+2.66Ⅳ。筛选出SRR适宜引物10对,明确了新品系及其亲本基间在DNA水平上的遗传差异,建立了能识别各材料育成登记的SSR指纹图。In order to clarify the molecular and cellular differences of five new potato strains,NNS-C 2,NNS-A 6,NNS-A 10,NNS-B 2 and NNS-A 11,which were selected from the F 1 segregating population of three potato hybirdized.This study conducted chromosome pairing configuration and SSR analysis of five new strains with the parents as control.Research showed that five new strains NNS-C 2,NNS-A 6,NNS-A 10,NNS-B 2 and NNS-A 11 chromosome pairing configurations had certain differences,which were 1.95Ⅰ+15.4Ⅱ+1.35Ⅲ+2.77Ⅳ,5.66Ⅰ+11.47Ⅱ+3.6Ⅲ+2.15Ⅳ,2.98Ⅰ+13.54Ⅱ+2.2Ⅲ+2.83Ⅳ,8.27Ⅰ+9.26Ⅱ+4.43Ⅲ+1.98Ⅳ,and 3.47Ⅰ+12.13Ⅱ+3.21Ⅲ+2.66Ⅳin turn.10 suitable primers of SRR were selected to clarify the genetic differences between the new strain and its parent base at the DNA level,and the SSR fingerprint that could identify the breeding registration of each material was established.

关 键 词：马铃薯新品系 SSR指纹 染色体构型 

分 类 号：S532[农业科学—作物学]