MAPK信号通路及STAT3、STAT5A/B在银屑病皮损中表达增高  

Over-expression of MAPK signaling pathway and STAT3 and STAT5A/B in psoriatic lesions

作　　者：刘凤杰 栗玉珍[1] LIU Fengjie;LI Yuzhen(Department of Dermatology,the Second Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第二医院皮肤科,哈尔滨150001

出　　处：《实用医学杂志》2018年第18期3020-3023,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81573042)

摘　　要：目的检测寻常型银屑病患者皮损中p38丝裂原活化蛋白激酶(p38 MAPK)、细胞外调节蛋白激酶(ERK1/2)、c-Jun氨基末端激酶(JNK)、丝氨酸/苏氨酸激酶(Akt)、蛋白S6激酶1(p70S6k)、核因子-кB(NF-кB)、信号传导及转录激活因子3(STAT3)、信号传导及转录激活因子5(STAT5A/B)、钙反应元件结合蛋白(CREB)的蛋白表达情况,探讨这些因子在银屑病中的作用。方法收集27例寻常型银屑病患者皮损(银屑病组)及19例健康者皮肤组织(对照组),应用高灵敏MILLIPLEX MAP试剂及Luminex仪检测并定量9种因子的蛋白表达,比较两组之间的表达水平。结果 p38、ERK1/2、JNK、STAT3、STAT5A/B在银屑病组的表达量高于对照组,差异均有统计学意义(均P <0.05);STAT5A/B与p38、ERK1/2、JNK、STAT3呈正相关(均P <0.05)。结论 JNK、p38、ERK1/2、STAT3、STAT5A/B在寻常型银屑病中表达增高,且STAT5A/B与p38、ERK1/2、JNK、STAT3有正相关性,表明STAT5A/B与MAPK因子互相作用,可能共同参与银屑病发病机制。Objective To detect the expression of p38mitogen-activated protein kinase(p38MAPK),extracellular regulated protein kinases1/2(ERK1/2),c-Jun N-terminal kinase(JNK),serine/threonine kinase(Akt),170 kDa ribosomal proteinS6 kinase(p70S6k),nuclear factor-кB(NF-кB),STAT3(signal transducers and activators of transcription 3),STAT5A/B(signal transducers and activators of transcription 5)andcyclic-AMP response binding protein(CREB)in psoriasispatients and to explore their roles in psoriasis.Methods The skin tissues were collected from 27 patients with psoriasis vulgaris and 19 normal controls.The highly sensitive MILLIPLEX MAP reagent and Luminex instrument were used to detectand quantitate the protein expression of MAPK signal pathway and the related factors.Results The expression of JNK,p38,ERK1/2,STAT3 and STAT5A/B in psoriasis vulgaris patients were significantly higher than that in the normal controls(P<0.05).STAT5A/B was positively correlated with p38,ERK1/2,JNK and STAT3(P<0.05).Conclusions The expression of JNK,p38,ERK1/2,STAT3 and STAT5A/B is over-expressed in psoriasis,and STAT5A/B has positive correlation with p38,ERK1/2,JNK and STAT3.All these factors interacting with each other may participate in the pathogenesis of psoriasis.

关 键 词：银屑病 MAPK信号转导通路 p38 ERK1/2 JNK STAT3 STAT5A/B 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]