乙醇诱导的柞蚕丝素蛋白多孔材料的研究  

Studies on Antheraea pernyi silk fibroin porous materials induced by ethanol

作　　者：王心如 邹盛之 冯文庆 张耀鹏[1,2] 张佳明 邵惠丽[1,2] WANG Xinru;ZOU Shengzhi;FENG Wenqing;ZHANG Yaopeng;ZHANG Jiamin;SHAO Huili(State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Shanghai 201620,China;College of Materials Science and Engineering,Donghua University,Shanghai 201620,China;Department of Geriatric Cardiology,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区：[1]东华大学纤维材料改性国家重点实验室,上海201620 [2]东华大学材料科学与工程学院,上海201620 [3]复旦大学附属华山医院老年科,上海200040

出　　处：《丝绸》2018年第10期1-8,共8页Journal of Silk

基　　金：国家自然科学基金项目(21674018);上海市曙光人才计划项目(15SG30);国家重点研发计划(2018YFC1105800;2018YFC1106002)

摘　　要：文章采用乙醇诱导再生柞蚕丝素蛋白(ASF)水溶液形成凝胶,并对该凝胶冷冻干燥制备出不溶于水的ASF多孔材料,利用傅里叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)研究乙醇水溶液浓度、ASF初始浓度对ASF凝胶及其冻干后样品结构的影响,并采用激光扫描共聚焦显微镜(LSCM)对ASF多孔材料的生物相容性进行评价。FTIR结果表明,乙醇诱导ASF水溶液成胶伴随着大量β-折叠构象的形成。SEM结果显示,冻干后的ASF多孔材料孔径在14.2~30. 4μm。LSCM结果表明,该材料在生物医学领域具有潜在的应用价值。Antheraeapernyi silk fibroin(ASF)gels were prepared from regenerated ASF aqueous solutions induced by ethanol,and freeze-dried to form insoluble ASF porous materials.Fourier transform infrared spectroscopy(FTIR)and scanning electron microscope(SEM)were used to investigate the effects of ethanol concentration and original ASF concentration on the structure of ASF gel and its freeze-dried samples.Moreover,laser scanning confocal microscope(LSCM)was used to further evaluate the biocompatibility of the obtained ASF porous materials.FTIR results showed that the gelation process of regenerated ASF aqueous solution induced by ethanol was accompanied by the formation of substantialβ-sheet conformation.SEM results indicated that the pore size of the obtained ASF freeze-dried samples ranged from 14.2 to 30.4μm.LSCM results further showed that the ASF materials had great potential for biomedical application.

关 键 词：柞蚕丝素蛋白 冷冻干燥 FTIR β-折叠构象 凝胶 

分 类 号：TS102.33[轻工技术与工程—纺织工程]