拟南芥AtBT4在抵抗Pst DC3000侵染过程中的功能  

Function analysis of AtBT4 gene fromArabidopsis against Pst DC3000

作　　者：郑旭[1] 黄聪聪[1] 庞茜[1] 藏金萍[1] 曹宏哲 张康 董金皋[1] 邢继红[1] ZHENG Xu;HUANG Cong-cong;PANG Xi;ZANG Jin-ping;CAO Hong-zhe;ZHANG Kang;DONG Jin-gao;XING Ji-hong(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/ Mycotoxin and Molecular Plant Pathology Laboratory,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学河北省植物生理与分子病理学重点实验室/真菌毒素与植物分子病理学实验室,河北保定071000

出　　处：《河北农业大学学报》2018年第5期32-36,共5页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(31200203);河北省自然科学基金(C2012204032);高等学校博士学科点专项科研基金(20121302120007)

摘　　要：为明确拟南芥AtBT4基因在抵抗Pst DC3000侵染过程中的功能,本研究检测AtBT4基因的T-DNA插入突变体bt4和bt4-1、回复突变体CE和超表达突变体OE对Pst DC3000的敏感性,结果发现,bt4、bt4-1突变体感病症状较为严重、病菌cfu值明显增强、胼胝质含量明显降低,表明AtBT4基因在拟南芥抵抗Pst DC3000侵染过程中发挥正调控的作用。利用Real-time PCR技术,检测野生型和突变体bt4、bt4-1、CE、OE接种Pst DC3000后SA、JA/ET信号途径关键基因ICS1、NPR1、JAR1、PDF1.2的表达情况,发现bt4和bt4-1突变体中各基因的表达水平显著低于野生型、回复突变体和超表达突变体,表明AtBT4基因正调控ICS1、NPR1、JAR1、PDF1.2的表达。由此推测,AtBT4基因通过调控SA、JA/ET信号途径影响拟南芥对Pst DC3000的抗性。研究结果为进一步阐明AtBT4基因调控拟南芥抵抗Pst DC3000侵染的分子机制奠定了良好基础。To investigate the function of AtBT4 gene of Arabidopsis against Pst DC3000,the sensitivity of AtBT4 gene T-DNA insertion mutant bt4 and bt4-1,the complemented transgenic plants CE,and the overexpression transgenic plants OE to Pst DC3000 were detected.It was found that the bt4 and bt4-1 mutants showed more severe than wild-type plants Col-0,CE,and OE.The cfu values of Pst DC3000 in bt4 and bt4-1 mutants were significantly higher than those in Col-0,CE,and OE.The callosum content in bt4 and bt4-1 mutants were significantly lower than that of Col-0,CE,and OE.These results indicated that AtBT4 was a positive regulator factor in resistance of Arabidopsis to Pst DC3000.The expression levels of ICS1,NPR1,JAR1,and PDF1.2 of SA and JA/ET signaling pathway were detected by Real-time PCR in Col-0,bt4,bt4-1,CE,and OE inoculated with Pst DC3000.It was found that the expression levels of ICS1,NPR1,JAR1 and PDF1.2 genes in the bt4 and bt4-1 mutants were significantly lower than those in Col-0,CE,and OE.These results indicated that the AtBT4 positively regulate the expression of ICS1,NPR1,JAR1 and PDF1.2.Therefore,the AtBT4 may be involved in the regulation of SA and JA/ET signaling pathways to affect the resistance of Arabidopsis to Pst DC3000.All the results would build the foundation for the molecular mechanism of disease resistance regulation in Arabidopsis.

关 键 词：拟南芥 AtBT4 PST DC3000 抗病信号途径 

分 类 号：S432.1[农业科学—植物病理学]