短枝型苹果的遗传分析及分子标记筛选  

Genetic studies and molecular markers screening of spur-type apple

作　　者：张朝红[1] 杨凤秋[1] 陈东玫[1] 赵同生[1] 赵国栋[1] 李扬[1] 赵永波[1] ZHANG Chao-hong;YANG Feng-qiu;CHEN Dong-mei;ZHAO Tong-sheng;ZHAO Guo-dong;LI Yang;ZHAO Yong-bo(Changli Institute of Pomology,Hebei Academy of Agricultural and Forestry Science,Changli 066600)

机构地区：[1]河北省农林科学院昌黎果树研究所,昌黎066600

出　　处：《河北农业大学学报》2018年第5期80-83,共4页Journal of Hebei Agricultural University

基　　金：河北省省级预算项目(F17R06007;F18R060018);河北省科技技术研究与发展计划(16226312D);现代农业产业技术体系建设专项资金(CARS-27)

摘　　要：为了初步揭示短枝型苹果的遗传机制,本文以‘新红星’和‘宫崎短枝富士’正反交分离群体、‘宫崎短枝富士’ב坂田津轻’的分离群体植株6397株为试材,开展短枝型苹果的遗传分析及分子标记筛选。结果表明,短枝型苹果的后代中,枝条长度、枝条基部粗度、成枝力、总芽数、短枝率和尖削度表现为超亲变异趋势,萌芽率和节间长度为超低亲变异的趋势;短枝型苹果由显性单基因控制,并存在显性上位效应;筛选到CHG02、CHO01和CH02C06与短枝型密切相关的SSR标记,其遗传距离分别为2.6,4.6和4.6cM。Inheritance and molecular markers of spur-type apple were investigated in 6397 seedlings derived from reciprocal crosses of‘Starkrimson’and‘Miyazaki spur’and cross of‘Miyazaki spur’and‘Sakata Tsugaru’.The results shown that the populations showed transgressive segregation on the length of the branch,the thickness of the base of the branches,the growth of the branches,the number of shoots,the number of shoots,and the acuity of the shoot.The germination rate and inter-node length,on the contrary,appeared ultra-low affinity.The spur-type apple is controlled by dominant single gene.Three pairs of SSR markers,CHG02,CHO01,and CH02C06,closely related to the short branch were selected.

关 键 词：苹果 杂种 短枝型 遗传 分子标记 

分 类 号：S661.1[农业科学—果树学]