17β雌二醇在诱导骨髓间充质干细胞分化过程中趋化作用的研究  

The study on chemotaxis of 17β estradiol in inducing bone marrow mesenchymal stem cells differentiation

作　　者：张雯碧 李雄 李路 孙晓溪 徐丛剑 Zhang Wenbi;Li Xiong;Li Lu(Obstetrics and Gynecology Hospital Affiliated to Fudan University,Shanghai Jiai Genetics&IVF Institute,Shanghai 200011)

机构地区：[1]复旦大学附属妇产科医院上海集爱遗传与不育诊疗中心,上海200011 [2]上海市女性生殖内分泌相关疾病重点实验室,上海200011

出　　处：《现代妇产科进展》2018年第10期753-757,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金青年项目资助(No:81501234)

摘　　要：目的:探讨17β雌二醇在骨髓间充质干细胞(BMSC)向子宫内膜上皮细胞(EEC)方向分化过程中的趋化作用及相关趋化因子的检测。方法:将BMSC和子宫内膜间质细胞(ESC)共培养,并分为四组:BMSC组,BMSC+17β雌二醇处理组,BMSC+ESC组,BMSC+ESC+17β雌二醇处理组。培养5天后,Transwell实验观察17β雌二醇的趋化作用,芯片技术分析培养液中趋化因子的表达情况。结果:迁移实验结果示,ESC可促进BMSC迁移,添加17β雌二醇后,BMSC的迁移能力增强。基因芯片检测发现,BMSC+ESC+17β雌二醇处理组中MIP-3b、I-TAC、MIG、SDF-1a、MDC、MIP-3a等趋化因子表达量明显增加。基因芯片进行Go富集分析与KEGG富集分析均发现,17β雌二醇显著增加细胞因子活性和细胞因子受体结合相关基因及信号通路差异表达的基因数量,从而提高BMSC的迁移能力。结论:17β雌二醇可能通过促进ESC分泌趋化因子,促进BMSC趋化迁移,促使BMSC迁移至适宜的微环境,最终定向分化为子宫内膜细胞。Objective:To investigate the chemotaxis of 17βestradiol in inducing bone marrow mesenchymal stem cells(BMSC)differentiation to endometrium epithelial cells(EEC)and to explore the specific cytokines.Methods:BMSC was co-cultured with endometrial stromal cell(ESC)and they were divided into four groups:BMSC,BMSC+17βestrodiol,BMSC+ESC,BMSC+ESC+17βestrodiol.The chemotaxis of BMSC in transwell experiment was performed after 5 days and the gene chip was analyzed for the cytokines in the culture media.Results:The migration ability of BMSC was enhanced with ESC and especially with both ESC and 17βestradiol.The gene chip result demonstrated that the expressions of MIP-3b,I-TAC,MIG,SDF-1a,MDC,MIP-3a were significantly increased in BMSC+ESC+17βestrodiol group.Go enrichment analysis and KEGG enrichment analysis found that 17βestradiol significantly increased the activity of cytokines and the number of genes expressed by cytokine receptor binding genes and signal pathways,thus improving the migration ability of BMSC.Conclusion:BMSC chemotactic migration is elevated by 17βestradiol through promoting ESC secreting the chemokines and finally the chemokines boost BMSC migrating to the appropriate microenvironment and differentiate into endometrium cells under a certain microenvironment.

关 键 词：雌激素 骨髓间充质干细胞 子宫内膜异位症 趋化 

分 类 号：R711.71[医药卫生—妇产科学]