大鼠毛囊干细胞体外无血清培养体系的建立  

Establishment of serum-free rat hair follicle stem cell culture system

作　　者：夏肖雪 吕正梅 张守兵 Xia Xiaoxue;Lü Zhengmei;Zhang Shoubing(Dept of Histology and Embryology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032;Central Laboratory of Molecular and Cellular Biology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学基础医学院组织胚胎学教研室,合肥230032 [2]安徽医科大学基础医学院分子和细胞生物学中心实验室,合肥230032

出　　处：《安徽医科大学学报》2018年第10期1631-1635,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽医科大学博士科研资助基金(编号:XJ201224);国家自然科学基金面上项目(编号:81471504)

摘　　要：建立大鼠毛囊干细胞的体外无血清培养体系。使用化学成分确定的Knock Out血清替代物(KSR)代替现有毛囊干细胞生长培养基中的胎牛血清,比较两种培养基培养的毛囊干细胞的形态特点和生长情况;通过毛囊重建实验证明该无血清培养基能够在体外维持毛囊干细胞的"干性";检测无血清培养基培养的毛囊干细胞中α6-整合素、角蛋白K14和P63的表达情况。毛囊干细胞在该无血清培养基中可正常生长并表达上述蛋白,将其移植到裸鼠背部,能够重建含毛囊皮肤。建立了大鼠毛囊干细胞的体外无血清培养体系并重建有毛皮肤,为毛囊干细胞的临床研究和应用提供了理论基础和技术支持。To establish a serum-free rat hair follicle stem cells(HFSCs)culture system.Fetal bovine serum(FBS)in the currently used HFSCs growth medium was replaced by chemically defined KnockOut serum replacement(KSR).Cell morphology and growth curve were compared between HFSCs cultured in the serum-free culture medium and FBS-containing culture medium.Hair follicle reconstitution experiment was conducted to validate whether stemness of HFSCs cultured in the serum-free culture medium was maintained.In addition,the expression ofα6-intergin,K14 and P63 in HFSCs cultured in the serum-free culture medium was detected by immunofluorescence staining.HFSCs cultured in the serum-free culture medium showed normal cell morphology and growth rate.Hair follicle-containing skin was reconstituted by HFSCs cultured under serum-free culture medium.Proteins includingα6-intergin,K14 and P63 highly expressed in HFSCs were still expressed in HFSCs cultured in serum-free culture medium.Serum-free rat HFSC culture medium was established.This provides the theoretical basis and technical support for the clinical research and application of HFSCs.

关 键 词：毛囊干细胞 无血清培养基 血清替代物 毛囊重建 

分 类 号：R644[医药卫生—外科学] Q813.1[医药卫生—临床医学]