黄缘闭壳龟微卫星分子标记的开发及遗传多样性分析  

Isolation and Genetic Diversity of Microsatellite Molecular Markers in the Yellow-margined Box Turtle( Cuora flavomarginata)

作　　者：荆胜利 张坤 黄小燕 张丽 黄斌[1,2] JING Shengli;ZHANG Kun;HUANG Xiaoyan;ZHANG Li;HUANG Bin(College of Life Sciences,Xinyang Normal University,Xinyang 464000,China;Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University,Xinyang 464000,China)

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]信阳师范学院大别山农业生物资源保护与利用研究院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2018年第4期555-561,共7页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31401732);信阳师范学院"南湖学者奖励计划"青年项目;大别山农业生物资源保护与利用学科群开放课题(2016)

摘　　要：采用FIASCO法构建了黄缘闭壳龟基因组微卫星富集文库,开发了18个具有多态性的微卫星分子标记. 18个位点的等位基因数目为2~14个不等,每个位点平均等位基因数为6.3个;观测杂合度在0.032和0.936之间变化(平均值为0.329),期望杂合度在0.148和0.903之间变化(平均值为0.635).黄缘闭壳龟微卫星分子标记的开发,将为其遗传学和种群结构分析研究提供有力的分子工具,并为制定新的保护策略提供理论依据.The 18 polymorphic microsatellite markers were developed and characterized for C.flavomarginata by using the Fast Isolation by AFLP of Sequence Containing Repeats(FIASCO)protocol to construct an enriched library of microsatellite.These markers revealed 2 to 14 alleles,with an average of 6.3 alleles per locus.The expected and observed heterozygosities ranged from 0.032 to 0.930(mean 0.329)and from 0.148 to 0.903(mean 0.635),respectively.Therefore,these markers would be useful tool for future genetics and population structure studies and establish new conservation strategies of C.flavomarginata.

关 键 词：黄缘闭壳龟 富集文库 微卫星 遗传多样性 

分 类 号：Q38[生物学—遗传学]