基于聚集诱导发光荧光探针的细胞成像研究进展  

Research Progress on Cell Imaging Based on the Aggregation-induced Emission Fluorescent Probes

作　　者：王涛[1] 马拉毛草 马恒昌[1] WANG Tao;MA Lamaocao;MA Hengchang(Key Laboratory of Polymer Materials of Gansu Province;Key Laboratory of Macromolecule Materials,Ministry of Education,College of Chemistry and Chemical Engineering,Lanzhou 730070,China;Shaw Library,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学化学与化工学院,生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,兰州730070 [2]西北师范大学逸夫图书馆,兰州730070

出　　处：《应用化学》2018年第10期1155-1165,共11页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(21764012)资助。

摘　　要：荧光探针是化学传感技术领域在20世纪末的一项重大发现,具有合成简单、灵敏度高、选择性好、响应时间短、可视化高等优点。将具有聚集诱导发光现象(AIE)特征的荧光基团与具有生物相容性的高分子结合起来,使得荧光材料具有毒性低、光稳定性好、生物相容性好等特点。在分子、离子检测和细胞成像技术中得到广泛的研究和应用。本文综述了细胞质成像、细胞膜成像、线粒体成像、溶酶体成像、脂滴成像、细胞核成像、细胞核和线粒体双靶向性成像的荧光探针,并对其应用前景做了展望。Fluorescent probes as a major discovery in the field of chemical sensing at the end of the 20th century have the advantages of simple synthesis,high sensitivity,good selectivity,short response time and high visualization.The combination of the fluorescent group with the aggregation-induced emission(AIE)characteristics and the biocompatible polymer makes the fluorescent materials have the characteristics of low toxicity,good light stability and good biocompatibility.In molecular,ion detection and cell imaging technology it has been widely studied and applied.This review summarizes the fluorescence probes for cytoplasmic imaging,cell membrane imaging,mitochondrial imaging,lysosomal imaging,lipid droplet imaging,nuclear imaging,nuclear and mitochondrial dual-targeting imaging and the prospects for their application.

关 键 词：荧光探针 化学传感 生物成像 聚集诱导发光 

分 类 号：O633[理学—高分子化学]