猪lncRNA-ENSSSCT00000018610的克隆及其在猪卵泡中的表达  

Cloning of Porcine lncRNA-ENSSSCT00000018610 and Its Expression Pattern in Porcine Ovarian Follicles

作　　者：李梦寻[1] 黄涛[1] 马力鹏[1] 刘乙[1] 李涛[1] 公红斌 邱梅玉 谢苏 孙晓梅[1] LI Meng-xun;HUANG Tao;MA Li-peng;LIU Yi;LI Tao;GONG Hong-bin;QIU Mei-yu;XIE Su;SUN Xiao-mei(College of Animal Science and Technology,Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学动物科技学院,石河子832000

出　　处：《畜牧兽医学报》2018年第9期1830-1839,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31460586)

摘　　要：为了探究lncRNA-ENSSSCT00000018610在猪卵泡发育过程中的作用,本研究采用RACE技术对lncRNA-ENSSSCT00000018610的全长序列进行了扩增克隆,用Real-time PCR进行组织表达谱分析,并分析其在梅山猪和杜洛克猪卵泡中的表达情况以及采用顺式和反式两种方法对lncRNA-ENSSSCT00000018610靶基因进行预测。结果表明,克隆获得了lncRNA-ENSSSCT00000018610全长序列1 731bp;lncRNA-ENSSSCT00000018610在肌肉、心、肝、脾、肺、肾、十二指肠、卵巢、输卵管、子宫、垂体、黄体、下丘脑中均有不同程度的表达,其中以肾、肺、子宫、卵巢表达量相对较高;lncRNA-ENSSSCT00000018610在杜洛克猪S、L卵泡中的表达量显著高于梅山猪(P<0.05),在杜洛克猪M1和M2卵泡中的表达水平极显著高于梅山猪(P<0.01)。lncRNA-ENSSSCT00000018610的潜在靶基因TNIP1、CYP2J2、SCARB1、IBSP等与猪产仔性状相关,并且参与猪的卵泡发育。提示其可能通过对靶基因的调控间接参与卵泡发育过程。To explore the role of lncRNA-ENSSSCT00000018610 in the development of porcine follicles,the full length RNA sequence of lncRNA-ENSSSCT00000018610 was amplified by RACE technique,it’s tissue expression profile and expression pattern in ovarian follicles of Meishan and Duroc sows were analyzed by Real-time PCR,and it’s target genes were predicted by using the cis and trans methods.A 1 731 bp-length sequence was obtained as the full length RNA sequence of lncRNA-ENSSSCT00000018610.lncRNA-ENSSSCT00000018610 was expressed differentially in variety of tissues(muscle,heart,liver,spleen,lung,kidney,duodenum,ovary,fallopian tube,uterus,pituitary,corpus luteum,hypothalamus),and expressed relatively higher in kidney,lung,uterus and ovary.The expression level of lncRNA-ENSSSCT00000018610 in S and L follicles of Duroc were significantly higher than that of Meishan(P<0.05).The expression level of lncRNA-ENSSSCT00000018610 in M1 and M2 follicles of Duroc were significantly higher than that of Meishan(P<0.01).The potential target genes of lncRNA-ENSSSCT00000018610 including TNIP 1,CYP 2J2,SCARB 1 and IBSP were related to the reproduction traits and participated in follicular development of pig.This result suggests that lncRNA-ENSSSCT00000018610 may participate in follicular development indirectly by regulating its target genes expression.

关 键 词：lncRNA-ENSSSCT00000018610 卵泡 表达量 母猪 

分 类 号：S828.2[农业科学—畜牧学]