广东省Ⅰ型鸭疫里氏杆菌分离鉴定及PFGE分型研究  

Analysis and PFGE Genotyping of SerotypeⅠRiemerella anatipestifer in Guangdong Province

作　　者：郭晶莹 李亚菲 蒋红霞[1] 曾振灵[1] GUO Jing-ying;LI Ya-fei;JIANG Hong-xia;ZENG Zhen-ling(South China Agricultural University/National Laboratory of Safety Evaluation in Guangdong Province,Guangzhou 510642,China;Public Monitoring Center for Agro-Product of Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]华南农业大学/国家兽药安全评价实验室,广州510642 [2]广东省农业科学院农产品公共监测中心,广州510640

出　　处：《畜牧兽医学报》2018年第9期1969-1978,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省农业科学院院长基金(201803);广东省自然科学基金研究团队项目(S2012030006590)

摘　　要：本文旨在研究广东地区Ⅰ型鸭疫里氏杆菌流行分布情况及其分子分型特点。2007—2017年,收集广东地区病死鸭、鹅的脑或肝组织等样品,进行菌株的分离、纯化及PCR鉴定等试验;采用玻片凝集的方法对鸭疫里氏杆菌进行Ⅰ型血清型鉴定,并进一步对血清Ⅰ型菌株进行脉冲场凝胶电泳(PFGE)分型,用BioNumerics 6.0软件进行聚类分析,比较同源性。结果表明:2007—2017年本实验室共分离保存220株鸭疫里氏杆菌,其中有119株为Ⅰ型血清型,占总分离株的54.09%。PFGE分型得到33种不同的PFGE分型图谱(以相似度≥85%为判断标准),可分为19个簇及14个单一型。血清Ⅰ型是广东地区鸭疫里氏杆菌的优势血清型。Ⅰ型鸭疫里氏杆菌分子型别呈现多样化,菌株之间的同源性差异较高,但存在小范围的克隆传播。The objective of this study was to investigate the prevalence of serotypeⅠRiemerella anatipestifer isolates in Guangdong province and obtain the molecular typing characteristics.R.anatipestifer strains were isolated from brain or liver samples collected from sick and dead ducks or geese during 2007 to 2017,and then these strains were subjected to purification and identification by PCR method.R.anatipestifer serotypeⅠwere identified by glass aggregation method and tested for pulsed field gel electrophoresis(PFGE)to analyze the homology by clustering analysis with BioNumerics 6.0 software.Results were as follows:A total of 220 R.anatipestifer isolates were collected in our laboratory during 2007 to 2017,and 119 R.anatipestifer isolates were serotypeⅠ,accounting for 54.09%of all isolated strains.PFGE genotyping showed that R.anatipestifer serotypeⅠisolates were differentiated into thirty-three different PFGE types(with similarity percentage of≥85%),including nineteen clusters and fourteen single types.In conclusion,the predominate serotype of R.anatipestifer isolates in Guangdong province was serotypeⅠ.SerotypeⅠR.anatipestifer isolates showed diverse genotyping band patterns.Low degree of homology was observed among these isolates and there was a clonal spread on a small scale.

关 键 词：鸭疫里氏杆菌 血清型 脉冲场凝胶电泳 

分 类 号：S852.61[农业科学—基础兽医学]