１５个北方杨树栽培品种倍性检测与ＳＳＲ遗传分析  

Ploidy Detection and Genetic Analysis of SSR Markers of 15 Populus Cultivars in North China

作　　者：张金旺 李代丽 尚峰男[3] 阿茹娜 铁龙 王君[3] 姜鹏 Zhang Jinwang;Li Daili;Shang Fengnan;A Runa;Tie Long;Wang Jun;Jiang Peng(Academy of Forestry in Tongliao City,Tongliao Inner Mongolia Autonomous Region 028000,China;Beijing luangfa Nurseiy,Beijing 102601,China;National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants/College of Biological Sciences and Technology,Beijing Forestry Lniversity,Beijing 100083,China)

机构地区：[1]内蒙古通辽市林业科学研究院,内蒙古通辽028000 [2]北京市黄垡苗圃,北京102601 [3]北京林业大学林木育种国家工程实验室,林木花丼遗传育种教育部重点实验室,生物科学与技术学院,北京100083

出　　处：《西南林业大学学报（自然科学）》2018年第5期27-33,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家重点研发计划课题(2016YFD0600404)资助；科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)(北京市级)(CEFF-PXM2017_014207_000043)资助。

摘　　要：为丰富杨树栽培品种的遗传信息,收集了中国北方地区广泛栽培的15个杨树栽培品种,在分析其倍性水平的基础上,利用毛细管电泳检测技术对这些品种进行了SSR遗传分析。结果表明:‘黑林1号杨’和‘白林3号杨’均为三倍体,表明在北方地区栽培的杨树品种中存在三倍体种质;利用筛选的12对引物共扩增出106个等位变异,多态性等位变异105个,特异性等位变异46个,多态性等位变异和特异性等位变异分别占99.06%和43.40%。依据各引物的等位位点特征,选择LG1926和LG1937这2对引物,可将15个品种进行有效区分,并为每个品种构建了SSR指纹图谱;采用非加权组平均法进行聚类分析,发现各品种间的相似系数为0.622 6～0.886 8,平均相似系数为0.733 7。1n this study,in order to enrich genetic information of Populus cultivars,15 cultivars widely used in North China were collected to detect their ploidy levels and to conduct genetic analysis based on SSR markers.1t was found that the cultivars P.x'Heilin 1'and P.X'Bailin 3'were triploids,suggesting that triploids were existed in Populus cultivars planted in North China.A total of 106 allelic variations were found from screened 12 pairs of SSR primers,including 105 polymorphic allelic variations and 46 specific variations,which accounted for 99.06%and 43.40%of total,respectively.According to characteristics of allelic variations for each pair of primer,the 15 cultivars could be effective distinguished by primer pairs LG1926 and LG1937.The SSR fingerprints were established for each cultivar.Clustering analysis with UPGMA(unweighted pair group method arithmetic averages)showed that the genetic similarities among cultivars ranged from 0.622 6 to 0.886 8,with average of 0.733 7.

关 键 词：杨树 品种 SSR 遗传分析 三倍体 

分 类 号：S792.11[农业科学—林木遗传育种]