西藏双须叶须鱼性腺发育的组织学观察（英文）  

HISTOLOGICAL STUDIES ON GONADAL DEVELOPMENT IN THE ENDEMIC TIBETAN FISH PTYCHOBARBUS DIPOGON

作　　者：曾本和 饶昌伟 刘海平 ZENG Ben-He;RAO Chang-Wei;LIU Hai-Ping(Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850002,China;Pangduo Township,Linzhou County,Lhasa 850000,China)

机构地区：[1]西藏自治区农牧科学院,水产科学研究所,拉萨850002 [2]西藏自治区拉萨市850000

出　　处：《水生生物学报》2018年第6期1194-1202,共9页Acta Hydrobiologica Sinica

基　　金：Supported by the Key R&D and Transformation Plans Tibet Autonomous Region Science and Technology Agency(XZ201801NB12);the Funds for Agricultural Science,Technology and Services of Tibet Autonomous Region Finance Department(2018SFTG01);the Special Finance of Tibet Autonomous Region(2017CZZX004,2017CZZX003;XZNKY-2018-C-040)

摘　　要：为丰富双须叶须鱼(Ptychobarbus dipogon)繁殖生物学,实验采用常规的石蜡切片及HE染色方法,对西藏双须叶须鱼的性腺发育及组织结构特征进行了研究,对双须叶须鱼人工繁殖成功具有重要的理论意义。结果表明:双须叶须鱼卵母细胞发育过程可分为5个时期,其卵巢发育可分为6个时期,在第Ⅴ期卵巢中,小卵和大卵数量比例为1.38鲶1,存在败育现象。雌性卵母细胞在第3时相卵黄颗粒和滤泡;第4时相卵母细胞中卵黄颗粒数量迅速增多,细胞核向动物极移动;第5时相卵母细胞中卵黄颗粒融合成片,卵细胞与滤泡膜分离并游离于卵巢腔中。双须叶须鱼精巢为小叶型,其生殖细胞可分为精原细胞、初级精母细胞、次级精母细胞、精子细胞和精子,其精巢发育也分为6个时期。双须叶须鱼属于分批同步产卵鱼类。The histology of gonadal development in Ptychobarbus dipogon was studied by using conventional paraffin sections and HE staining,in order to obtain information on its reproductive systems useful for its artificial breeding.The results showed that the development of oocytes can be divided into five phases,and ovarian development can be divided into six stages.In stage V ovaries,ovum worship was found to occur,indicated by the 1.38鲶1 ratio of small ova to large ova.Phase 3 oocytes were characterized by appearance of yolk granules and follicles;phase 4 oocytes by rapid increase of yolk granules,and the movement of the nucleus toward the animal poles;phase 5 by the fusion of yolk granules,and the separation of oocytes from follicular membranes to float in the ovarian cavity.Male P.dipogen had lobular testes,and their germ cells can be categorized into spermatogonia,spermatocytes;spermatids;and spermatozoa.Their testes also developed in six stages.The species followed a batch-synchronous spawning pattern.

关 键 词：双须叶须鱼 性腺发育 组织结构 产卵类型 

分 类 号：S917[农业科学—水产科学]